MER1, A YEAST GENE REQUIRED FOR CHROMOSOME-PAIRING AND GENETIC-RECOMBINATION, IS INDUCED IN MEIOSIS
MER1, A YEAST GENE REQUIRED FOR CHROMOSOME-PAIRING AND GENETIC-RECOMBINATION, IS INDUCED IN MEIOSIS
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DOI：
10.1128/mcb.10.5.2379
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发表时间：
1990-05-01
影响因子：
5.3
通讯作者：
ROEDER, GS
中科院分区：
文献类型：
--
作者：
ENGEBRECHT, J;ROEDER, GS
酵母MER 1基因是生产有活力的减数分裂产物和减数分裂重组所必需的。细胞学分析的染色体扩散从MER 1突变体表明，MER 1基因产物也需要正常的染色体配对。mer 1菌株产生轴向元件，联会复合体的前体;然而，大多数细胞核中的染色体不完全联会。确定MER 1编码区的DNA序列; MER 1开放阅读框编码270个氨基酸的蛋白质，分子量为31.1千道尔顿。MER 1蛋白显示出与钙调蛋白有限的序列相似性。通过RNA印迹杂交分析和mer 1：：lacZ融合基因的构建和分析来检测MER 1基因的表达。MER 1基因的表达是减数分裂诱导的，并且需要IME 1基因产物。因此，MER 1基因在减数分裂早期的表达是正确的染色体配对和减数分裂重组所必需的。
The yeast MER1 gene is required for the production of viable meiotic products and for meiotic recombination. Cytological analysis of chromosome spreads from a mer1 mutant indicates that the MER1 gene product is also required for normal chromosome pairing. mer1 strains make axial elements, precursors to the synaptonemal complex; however, the chromosomes in most nuclei do not become fully synapsed. The DNA sequence of the MER1 coding region was determined; the MER1 open reading frame encodes a 270-amino-acid protein with a molecular mass of 31.1 kilodaltons. The MER1 protein shows limited sequence similarity to calmodulin. Expression of the MER1 gene was examined by RNA blot hybridization analysis and through the construction and analysis of mer1::lacZ fusion genes. Expression of the MER1 gene is meiotically induced and required the IME1 gene product. Thus, expression of the MER1 gene early in meiosis is required for proper chromosome pairing and meiotic recombination.