Mechanical Control of Morphogenesis by Fat/Dachsous/Four-Jointed Planar Cell Polarity Pathway
Mechanical Control of Morphogenesis by Fat/Dachsous/Four-Jointed Planar Cell Polarity Pathway
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DOI：
10.1126/science.1221071
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发表时间：
2012-05-11
期刊：
影响因子：
56.9
通讯作者：
Bellaiche, Yohanns
中科院分区：
文献类型：
--
作者：
Bosveld, Floris;Bonnet, Isabelle;Bellaiche, Yohanns
在动物发育过程中，几个平面细胞极性（PCP）途径通过协调集体细胞行为来控制组织形状。在这里，我们的特点是通过多尺度成像上皮形态发生在果蝇背胸，并显示如何脂肪/Dachsous/四关节PCP通路控制形态发生。我们发现，原钙粘蛋白Dachsous是极化的一个域的组织范围内的表达梯度。此外，Dachsous极化肌球蛋白Dachs，这反过来又促进连接张力的各向异性。通过将物理建模与定量图像分析相结合，我们确定了这种张力各向异性定义了局部组织收缩的模式，该模式主要通过定向细胞重排来塑造上皮。我们的研究结果建立了组织平面极化如何协调细胞力学特性的局部变化来控制组织形态发生。
During animal development, several planar cell polarity (PCP) pathways control tissue shape by coordinating collective cell behavior. Here, we characterize by means of multiscale imaging epithelium morphogenesis in the Drosophila dorsal thorax and show how the Fat/Dachsous/Four-jointed PCP pathway controls morphogenesis. We found that the proto-cadherin Dachsous is polarized within a domain of its tissue-wide expression gradient. Furthermore, Dachsous polarizes the myosin Dachs, which in turn promotes anisotropy of junction tension. By combining physical modeling with quantitative image analyses, we determined that this tension anisotropy defines the pattern of local tissue contraction that contributes to shaping the epithelium mainly via oriented cell rearrangements. Our results establish how tissue planar polarization coordinates the local changes of cell mechanical properties to control tissue morphogenesis.