Diverse sensitivities of TRPA1 from different mosquito species to thermal and chemical stimuli
Diverse sensitivities of TRPA1 from different mosquito species to thermal and chemical stimuli
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DOI：
10.1038/s41598-019-56639-w
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发表时间：
2019-12-27
影响因子：
4.6
通讯作者：
Tominaga, Makoto
中科院分区：
文献类型：
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作者：
Li, Tianbang;Saito, Claire T.;Tominaga, Makoto
温度和气味深刻地影响着动物的行为。瞬时受体电位通道，亚家族A，成员1（TRPA 1）作为一种多模态伤害感受器，在昆虫中感受两种重要的环境线索。蚊子是一种重要的疾病传播媒介，人们对其寄主寻找行为和驱避剂进行了大量的研究。然而，蚊子TRPA1的生理特性尚未得到系统的研究。我们确定了多种选择性剪接变体的TrpA1基因冈比亚按蚊，斯氏按蚊，埃及伊蚊和库蚊pipiens pallens蚊子。并采用钙离子成像和全细胞膜片钳技术对蚊TRPA1对热刺激和化学刺激的反应进行了比较分析。不同热生境的4种蚊TRPA1的比较结果表明，温带淡色库蚊TRPA1具有较低的热激活温度阈值，这一结果得到了避热实验的支持。值得注意的是，蚊子TRPA1通道的化学敏感性不仅在变体之间而且在物种之间显示出差异。此外，我们发现了三种新的蚊子TRPA1激动剂。热生态位的选择和进化轨迹显着影响蚊子TRPA1的功能特性，这代表了一个标志的行为，可以允许设计改进的蚊子控制方法。
Temperature and odors profoundly affect the behavior of animals. Transient receptor potential channel, subfamily A, member 1 (TRPA1) functions as a polymodal nociceptor for sensing both vital environmental cues in insects. Mosquitoes are recognized as disease vectors, and many efforts have been devoted to investigations of their host-seeking behaviors and repellents. However, the physiological characteristics of mosquito TRPA1 have not been systematically studied. We identified multiple alternative splice variants of the TrpA1 gene from Anopheles gambiae, Anopheles stephensi, Aedes aegypti and Culex pipiens pallens mosquitoes. And we performed comparative analyses of the responses of mosquito TRPA1s to heat or chemical stimuli with calcium-imaging and whole-cell patch-clamp methods. Comparison of TRPA1 among four mosquito species from different thermal niches revealed that TRPA1 of Culex pipiens pallens inhabiting the temperate zone had a lower temperature threshold for heat-evoked activation, which was supported by the in vivo heat-avoidance test. Notably, the chemosensitivity of mosquito TRPA1 channels revealed differences not only between variants but also among species. Moreover, we discovered three novel mosquito TRPA1 agonists. Thermal niches selection and evolutionary trajectories significantly affect the functional properties of mosquito TRPA1, which represents a hallmark of the behaviors that may permit the design of improved mosquito control methods.