Quantitative assessment of Malaysian Plasmodium falciparum clones to schizontocidal drugs before and after cryopreservation
Quantitative assessment of Malaysian Plasmodium falciparum clones to schizontocidal drugs before and after cryopreservation
复制标题
DOI：
10.1159/000007238
复制
发表时间：
1999-11-01
期刊：
影响因子：
3.3
通讯作者：
Cheang, HS
中科院分区：
文献类型：
--
作者：
Ang, HH;Cheang, HS
使用有限稀释法从五种马来西亚恶性疟原虫分离株中获得了三十个克隆。然后使用改良的体外微技术对这些克隆进行抗疟测试。结果显示，冷冻保存后，ST 85/B3、GC/C10和ST 85/A2克隆对氯喹、甲氟喹和奎宁的敏感性分别降低至19%、41%和28%，而ST 12/F8、ST 85/B3和ST 85/B3克隆对氯喹、甲氟喹和奎宁的敏感性分别增加6%、43%和21%。进一然而，它们在冷冻保存后仍保持其原始敏感性。版权所有 (C) 1999 S. Karger AG，巴塞尔。
Thirty clones were obtained from five Malaysian Plasmodium falciparum isolates using the limiting dilution method. These clones were then subjected to antimalarial testing using the modified in vitro microtechnique. The results showed that ST 85/B3, GC/C10 and ST 85/A2 clones decreased their susceptibilities to 19, 41 and 28% whilst ST 12/F8, ST 85/B3 and ST 85/B3 clones showed increases of 6, 43 and 21%, respectively, against chloroquine, mefloquine and quinine after cryopreservation. Further results also indicated that GC/B4, GC/B7, GC/C10, ST 85/A5, ST 85/ D3, ST 148/F8 clones did not show any change (up to 2 decimal places) against chloroquine, ST 12/D5, ST 12/E8, ST 12/F8, ST 148/A5 clones against quinine after cryopreservation. They, however, maintained their original susceptibilities after cryopreservation. Copyright (C) 1999 S. Karger AG, Basel.