A Genomic Approach to Yeast Chronological Aging
A Genomic Approach to Yeast Chronological Aging
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DOI：
10.1007/978-1-59745-540-4_6
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Kaeberlein, Matt
中科院分区：
文献类型：
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作者：
Burtner, Christopher R.;Murakami, Christopher J.;Kaeberlein, Matt
酵母是研究衰老的遗传和生化机制的有用模式生物。然而，酵母衰老的基因组研究受到传统方法的限制，这些方法需要大量的人力和资源投入。在本章中，我们描述了一种新开发的方法，用于定量测量酵母ORF缺失集合中包含的每个菌株的时间寿命。我们的方法包括通过使用Bioscreen C MBR振荡器/培养箱/读板器监测生长动力学来确定种群存活。该方法具有与传统测定法相当的准确性，同时允许更高的通量和降低的测量变异性。
Yeast is a useful model organism to study the genetic and biochemical mechanisms of aging. Genomic studies of aging in yeast have been limited, however, by traditional methodologies that require a large investment of labor and resources. In this chapter, we describe a newly-developed method for quantitatively measuring the chronological life span of each strain contained in the yeast ORF deletion collection. Our approach involves determining population survival by monitoring outgrowth kinetics using a Bioscreen C MBR shaker/incubator/plate reader. This method has accuracy comparable to traditional assays, while allowing for higher throughput and decreased variability in measurement.