Dietary fish oil modulates macrophage fatty acids and decreases arthritis susceptibility in mice.
Dietary fish oil modulates macrophage fatty acids and decreases arthritis susceptibility in mice.
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DOI：
10.1084/jem.162.4.1336
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发表时间：
1985-10-01
期刊：
影响因子：
--
通讯作者：
Cathcart ES
中科院分区：
文献类型：
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作者：
Leslie CA;Gonnerman WA;Ullman MD;Hayes KC;Franzblau C;Cathcart ES
B10.RIII和B10.G小鼠从实验鼠饲料中转移到标准饲料中，所有脂肪(重量百分比为5%)以鱼油(17%二十碳五烯酸[EPA]，12%二十二碳六烯酸[DHA]，0%花生四烯酸[AA]和2%亚油酸)或玉米油(0%EPA，0%DHA，0%AA和65%亚油酸)补充。饲喂饲料的小鼠巨噬细胞磷脂的脂肪酸组成与食用玉米油的小鼠相似。与饲喂玉米油的小鼠(0%EPA、0%DPA、6%DHA、20%AA、9%DTA和8%亚油酸)相比，仅喂食1 wk鱼油的小鼠巨噬细胞内omega-3脂肪酸(总脂肪酸中4%为EPA、10%的二十二碳五烯酸[DPA]和10%的DHA)的磷脂水平显著增加，而omega-6脂肪酸(12%的AA、2%的二十二碳四烯酸[DTA]和4%的亚油酸)显著降低。5周后，喂鱼油的老鼠和喂玉米油的老鼠之间的这种差异更加明显。在5-9wk时出现进一步的微小变化。我们研究了在胶原蛋白免疫前饲喂这两种饲料的小鼠的巨噬细胞的前列腺素(PG)和血栓素(TX)的分布。无论饮食如何，孵育24小时的雌性小鼠的巨噬细胞在培养液中的PG和TX显著高于雄性小鼠的类似制备的巨噬细胞。饲喂鱼油的小鼠巨噬细胞磷脂中EPA百分含量的增加和AA百分含量的降低，反映在培养液中前列腺素E_2和前列腺素I_2的含量比相同孵育的玉米油饲喂小鼠的巨噬细胞减少。与饲喂玉米油的B10.RIII小鼠相比，B10.RIII小鼠在II型胶原蛋白免疫前喂饲相同的鱼油饲料26d，关节炎的发病时间延长，关节炎的发生率和严重程度降低。女性，尤其是那些食用鱼油的女性，患关节炎的几率往往比男性要低。这些可用于PG和白三烯合成的脂肪酸库的变化表明，巨噬细胞和PG在II型胶原的免疫和/或炎症反应中起着关键作用。
B10.RIII and B10.G mice were transferred from a diet of laboratory rodent chow to a standard diet in which all the fat (5% by weight) was supplied as either fish oil (17% eicosapentaenoic acid [EPA], 12% docosahexaenoic acid [DHA], 0% arachidonic acid [AA], and 2% linoleic acid) or corn oil (0% EPA, 0% DHA, 0% AA, and 65% linoleic acid). The fatty acid composition of the macrophage phospholipids from mice on the chow diet was similar to that of mice on a corn oil diet. Mice fed the fish oil diet for only 1 wk showed substantial increases in macrophage phospholipid levels of the omega-3 fatty acids (of total fatty acid 4% was EPA, 10% docosapentaenoic acid [DPA], and 10% DHA), and decreases in omega-6 fatty acids (12% was AA, 2% docosatetraenoic acid [DTA], and 4% linoleic acid) compared to corn oil-fed mice (0% EPA, 0% DPA, 6% DHA, 20% AA, 9% DTA, and 8% linoleic acid). After 5 wk this difference between the fish oil-fed and corn oil-fed mice was even more pronounced. Further small changes occurred at 5-9 wk. We studied the prostaglandin (PG) and thromboxane (TX) profile of macrophages prepared from mice fed the two diets just before being immunized with collagen. Irrespective of diet, macrophages prepared from female mice and incubated for 24 h had significantly more PG and TX in the medium than similarly prepared macrophages from male mice. The increased percentage of EPA and decreased percentage of AA in the phospholipids of the macrophages prepared from the fish oil-fed mice was reflected in a reduction in the amount of PGE2 and PGI2 in the medium relative to identically incubated macrophages prepared from corn oil-fed mice. When this same fish oil diet was fed to B10.RIII mice for 26 d before immunization with type II collagen, the time of onset of arthritis was increased, and the incidence and severity of arthritis was reduced compared to arthritis induced in corn oil-fed mice. The females, especially those on the fish oil diet, tended to have less arthritis than the males. These alterations in the fatty acid pool available for PG and leukotriene synthesis suggest a pivotal role for the macrophage and PG in the immune and/or inflammatory response to type II collagen.