Berberine ameliorates hyperglycemia in alloxan-induced diabetic C57BL/6 mice through activation of Akt signaling pathway.
Berberine ameliorates hyperglycemia in alloxan-induced diabetic C57BL/6 mice through activation of Akt signaling pathway.
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DOI：
10.1507/endocrj.k11e-024
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发表时间：
2011
影响因子：
2
通讯作者：
Xi Xie;Wenyuan Li;Tianyun Lan;Weihua Liu;Jing Peng;Kaipeng Huang;Juan Huang;Xiaoyan Shen;
中科院分区：
文献类型：
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作者：
Xi Xie;Wenyuan Li;Tianyun Lan;Weihua Liu;Jing Peng;Kaipeng Huang;Juan Huang;Xiaoyan Shen;
近年来，有研究表明Akt信号通路异常参与了糖尿病的病理过程。以往的研究表明，黄连素可通过促进肝糖原合成来降低血糖。然而，潜在的机制仍不清楚。本研究观察了黄连素对四氧嘧啶糖尿病小鼠的空腹血糖、肝糖原、Akt、糖原合成酶-3、葡萄糖激酶和胰岛素受体底物(IRS)的影响，并探讨其可能的降血糖机制。我们发现，在四氧嘧啶诱导的糖尿病小鼠中，黄连素治疗显著降低了高血糖。糖尿病小鼠肝糖原含量、葡萄糖激酶的表达和活性以及磷酸化Akt和IRS均显著降低，而黄连素可阻断这些变化。黄连素还能抑制糖尿病小鼠磷酸化GSK-3β的增加。总的来说，黄连素可能通过胰岛素信号通路上调Akt的活性，最终降低四氧嘧啶诱导的糖尿病小鼠的高血糖。
Recently, it is implicated that the abnormality of Akt signaling pathway is involved in the diabetic pathology. Previous studies have demonstrated that berberine could decrease blood glucose by elevating liver glycogen synthesis. However, the underlying mechanism is still unclear. In the present study, we investigated the effects of berberine on fasting blood glucose, liver glycogen, Akt, Glycogen synthase kinase-3, glucokinase and insulin receptor substrate (IRS) in alloxan-induced diabetic mice, exploring its possible hypoglycemic mechanism. We found that in alloxan-induced diabetic mice, the high blood glucose was significantly lowered by berberine treatment. Liver glycogen content, the expression and activity of glucokinase and the phosphorylated Akt and IRS were all significantly reduced in diabetic mice whereas berberine blocked these changes. Berberine also depressed the increasing of phosphorylated GSK-3β in diabetic mice. Collectively, Berberine upregulates the activity of Akt possibly via insulin signaling pathway, eventually lowering high blood glucose in alloxan-induced diabetic mice.