DISTRIBUTION AND POSSIBLE ROLE OF PERINUCLEAR THECA PROTEINS DURING BOVINE SPERMIOGENESIS
DISTRIBUTION AND POSSIBLE ROLE OF PERINUCLEAR THECA PROTEINS DURING BOVINE SPERMIOGENESIS
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DOI：
10.1002/jemt.1070320605
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发表时间：
1995-12-15
影响因子：
2.5
通讯作者：
MARAVEI, D
中科院分区：
文献类型：
--
作者：
OKO, R;MARAVEI, D
核周膜（PT）是包裹哺乳动物精子核的独特细胞骨架元件。分为顶体下区和顶体后区。本研究的目的是分析和阶段性的细胞内分布的几个promiment公牛PT蛋白在精子发生过程中。为此目的，使用针对15.5-、25-、28-、32-、36-和60-kDa公牛PT多肽产生并亲和纯化的多克隆抗体作为固定睾丸切片上的探针。免疫过氧化物酶染色显示，PT多肽首先出现在精子发生的早期，伴随着早期的顶体系统的发展步骤。免疫金标记进一步表明，这些多肽与整个顶体膜的外周相关，在精子细胞核上的顶体囊泡的附件之前和期间。一旦顶体覆盖在核上，标记主要位于精子细胞的顶体下区域，位于顶体内膜和核膜之间。后来，在精子细胞核的伸长过程中，除了抗15.5-kDa抗体的标记延伸到尾部的组装后顶体鞘。本研究表明，核周膜蛋白在精子细胞顶体与核的附着、伸展和结合过程中起着重要作用。(C)1995 Wiley-Liss，Inc.
The perinuclear theca (PT) is a unique cytoskeletal element that encapsulates the mammalian sperm nucleus. It is divided into subacrosomal and postacrosomal regions. The objective of this study was to analyze and stage the intracellular distribution of several promiment bull PT proteins during spermatogenesis. For this purpose, polyclonal antibodies raised and affinity-purified against the 15.5-, 25-, 28-, 32-, 36-, and 60-kDa bull PT polypeptides were used as probes on sections of aldehyde-fixed testes. Immunoperoxidase staining revealed that the PT polypeptides first appeared early in spermiogenesis, concomitant with early steps of development of the acrosomic system. Immunogold labeling further showed that these polypeptides were peripherally associated with the entire acrosomal membrane, before and during the attachment of the acrosomic vesicle onto the spermatid's nucleus. Once the acrosome had capped the nucleus the labeling resided mainly in the subacrosomal region of the spermatid, between the inner acrosomal membrane and nuclear envelope. Later, during the elongation of the spermatid's nucleus, the labeling with all antibodies except the anti-15.5-kDa antibody extended caudally over the assembling post-acrosomal sheath. This study suggests that the perinuclear theca proteins play an instrumental role in the attachment, spreading, and binding of the acrosome onto the nucleus of spermatids. (C) 1995 Wiley-Liss, Inc.