Activation of spinal kainate receptors after inflammation: behavioral hyperalgesia and subunit gene expression
Activation of spinal kainate receptors after inflammation: behavioral hyperalgesia and subunit gene expression
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DOI：
10.1016/s0014-2999(02)02333-6
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发表时间：
2002-10-11
影响因子：
5
通讯作者：
Ren, K
中科院分区：
文献类型：
--
作者：
Guo, W;Zou, SP;Ren, K
我们确定了对炎症和痛觉过敏的神经反应是否涉及红藻氨酸受体（谷氨酸受体的一个亚组）的激活。通过足底内注射完全弗氏佐剂将炎症引入结合爪。通过鞘内施用非选择性α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）/红藻氨酸受体拮抗剂、1,2,3,4-四氢-6-体外-2,3-二氧代-苯并[f]喹喔啉-7-磺酰胺二钠（NBQX）以及选择性红藻氨酸受体拮抗剂，减轻炎症诱导的热痛觉过敏。 6,7,8,9-四氢-5-体外-1H-苯并[g]吲哚-2,3-二酮3-肟 (NS-102) 和 3S,4aR,6S,8aR-6-(4-羧基苯基)甲基-1,2,3,4,4a,5,6,7,8,8a-十-氢异喹啉-3-羧酸（LY382884）。逆转录聚合酶链反应 (RT-PCR) 表明，GluR5 和 GluR6，而非 GluR7、KA1 和 KA2 亚基，在炎症后 2 小时至 3 天表现出 mRNA 表达增加（P
We determined whether neural responses to inflammation and hyperalgesia involve activation of kainate receptors, a subgroup of glutamate receptors. Inflammation was introduced into the bind paw by intraplantar injection of complete Freund's adjuvant. The inflammation-induced thermal hyperalgesia was attenuated by intrathecal administration of a non-selective alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate receptor antagonist, 1,2,3,4-tetrahydro-6-vitro-2,3-dioxo-benzo[f]quinoxaline-7-sulfonamide disodium (NBQX), as well as by selective kainate receptor antagonists, 6,7,8,9-tetrohydro-5-vitro-1H-benz[g]indole-2,3-dione 3-oxime (NS-102) and 3S,4aR,6S,8aR-6-(4-carboxyphenyl)methyl-1,2,3,4,4a,5,6,7,8,8a-deca-hydroisoquinoline-3-carboxylic acid (LY382884). Reverse transcription-polymerase chain reaction (RT-PCR) indicated that the GluR5 and GluR6, but not the GluR7, KA1 and KA2 subunits, exhibited increased mRNA expression at 2 h to 3 days following inflammation (P