Electrochemical evaluation of poly(3,4-ethylenedioxythiophene) films doped with bacteria based on viability analysis
Electrochemical evaluation of poly(3,4-ethylenedioxythiophene) films doped with bacteria based on viability analysis
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DOI：
10.1016/j.bioelechem.2015.05.003
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发表时间：
2015-10-01
影响因子：
5
通讯作者：
Nagaoka, Tsutomu
中科院分区：
文献类型：
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作者：
Dung Quynh Le;Tokonami, Shiho;Nagaoka, Tsutomu
为了将活细菌固定在电极上，我们提出了一种新颖且简单的技术，该技术依赖于细菌的负 zeta 电位作为掺杂剂插入导电聚合物中。在本研究中，我们对掺杂有各种革兰氏阴性细菌（包括铜绿假单胞菌、大肠杆菌和希瓦氏菌）的聚（3,4-乙撑二氧噻吩）（PEDOT）进行了电化学聚合。掺杂细菌的PEDOT薄膜表现出典型的氧化还原反应、高电导率和电化学稳定性。荧光显微镜证实，大约 90% 掺入厚度 >0.5 μm 的 PEDOT 薄膜中的细菌是有活力的。 (C) 2015 Elsevier B.V. 保留所有权利。
To immobilize viable bacteria on an electrode, we present a novel and straightforward technique that relies on the negative zeta-potentials of bacteria for insertion into conducting polymers as dopants. In the present study, we conducted an electrochemical polymerization of poly(3,4-ethylenedioxythiophene) (PEDOT) doped with various gram-negative bacteria, including Pseudomonas aeruginosa, Escherichia coli, and Shewanella oneidensis. The PEDOT film doped with bacteria indicated a typical redox response, high conductivity, and electrochemical stability. Fluorescence microscopy confirmed that approximately 90% of the bacteria incorporated into the PEDOT film at >0.5 mu m in thickness were viable. (C) 2015 Elsevier B.V. All rights reserved.