Functional evaluation of the insulin/insulin‐like growth factor signaling pathway in determination of wing polyphenism in pea aphid
Functional evaluation of the insulin/insulin‐like growth factor signaling pathway in determination of wing polyphenism in pea aphid
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DOI：
10.1111/1744-7917.13121
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发表时间：
2022-09
期刊：
影响因子：
4
通讯作者：
Yiyang Yuan;Yanyan Wang;W. Ye;Erliang Yuan;Jian Di;X. Chen;Yanling Xing;Yucheng Sun;F. Ge
中科院分区：
文献类型：
--
作者：
Yiyang Yuan;Yanyan Wang;W. Ye;Erliang Yuan;Jian Di;X. Chen;Yanling Xing;Yucheng Sun;F. Ge
翅多表型是昆虫的一种普遍现象，在昆虫的环境适应中起着重要作用。胰岛素/胰岛素样生长因子信号通路在后生动物的代谢、发育和生长调控中是一个高度保守的通路。据报道，褐飞虱通过调节翼垫的发育来改变翅膀形态需要IIS。然而，IIS通路是否以及如何调控蚜虫的跨代翅膀二态性仍然是一个谜。本研究发现，配对处理和独居处理分别能诱导豌豆蚜虫产生高比例和低比例的有翅子代。与配对相比，单独处理时母亲头部ILP5（胰岛素样肽5）的表达水平显著升高，而ILP5沉默对羽翼后代比例无明显影响。RNA干扰介导的FoxO（叉头转录因子O亚群）在20期胚胎中的敲低显著增加了有翼后代的比例。药理和定量聚合酶链反应实验结果表明，胚胎胰岛素受体可能不参与翅膀多酚现象。ILP4和ILP11在无翅子代中的表达量高于有翅子代。我们证明FoxO负向调控胚胎中翅膀形态的发育。ILPs可能以特定发育阶段的方式调节蚜虫翅膀多表型。然而，这种调节可能不是由规范的IIS途径介导的。这一发现促进了我们对昆虫跨代翅多表型IIS通路的理解。
Wing polyphenism is a common phenomenon that plays key roles in environmental adaptation of insects. Insulin/insulin‐like growth factor signaling (IIS) pathway is a highly conserved pathway in regulation of metabolism, development, and growth in metazoans. It has been reported that IIS is required for switching of wing morph in brown planthopper via regulating the development of the wing pad. However, it remains elusive whether and how IIS pathway regulates transgenerational wing dimorphism in aphid. In this study, we found that pairing and solitary treatments can induce pea aphids to produce high and low percentage winged offspring, respectively. The expression level of ILP5 (insulin‐like peptide 5) in maternal head was significantly higher upon solitary treatment in comparison with pairing, while silencing of ILP5 caused no obvious change in the winged offspring ratio. RNA interference‐mediated knockdown of FoxO (Forkhead transcription factor subgroup O) in stage 20 embryos significantly increased the winged offspring ratio. The results of pharmacological and quantitative polymerase chain reaction experiments showed that the embryonic insulin receptors may not be involved in wing polyphenism. Additionally, ILP4 and ILP11 exhibited higher expression levels in 1st wingless offspring than in winged offspring. We demonstrate that FoxO negatively regulates the wing morph development in embryos. ILPs may regulate aphid wing polyphenism in a developmental stage‐specific manner. However, the regulation may be not mediated by the canonical IIS pathway. The findings advance our understanding of IIS pathway in insect transgenerational wing polyphenism.