Nanophotonic Waveguide Enhanced Raman Spectroscopy of Biological Submonolayers
Nanophotonic Waveguide Enhanced Raman Spectroscopy of Biological Submonolayers
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DOI：
10.1021/acsphotonics.6b00593
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发表时间：
2016-11-01
期刊：
影响因子：
7
通讯作者：
Baets, Roel
中科院分区：
文献类型：
--
作者：
Dhakal, Ashim;Wuytens, Pieter C.;Baets, Roel
表征单层生物分子一直是一个重大挑战。我们展示了近红外区域单层的纳米光子波导增强拉曼光谱 (NWERS)，能够实时测量 DNA 链的杂交以及固定在光子芯片顶部的生物素-链霉亲和素复合物的亚单层密度。 NWERS 基于增强的倏逝激发和纳米光子波导附近自发拉曼散射的收集，对于 1 厘米氮化硅波导，其提供的信号比共焦拉曼显微镜高出 4 个数量级以上。减少的信号采集时间和特异性可以对表面物质进行定量和实时表征，这是迄今为止使用拉曼光谱无法实现的。 NWERS 为单层研究提供了直接分析工具，并为紧凑型无显微镜芯片实验室设备开辟了一条途径，该设备具有使用可大规模生产的互补金属氧化物半导体技术平台制造的集成源、光谱仪和探测器。
Characterizing a monolayer of biological molecules has been a major challenge. We demonstrate nanophotonic waveguide enhanced Raman spectroscopy (NWERS) of monolayers in the near-infrared region, enabling real-time measurements of the hybridization of DNA strands and the density of submonolayers of biotin-streptavidin complex immobilized on top of a photonics chip. NWERS is based on enhanced evanescent excitation and collection of spontaneous Raman scattering near nanophotonic waveguides, which for a 1 cm silicon nitride waveguide delivers a signal that is more than 4 orders of magnitude higher in comparison to a confocal Raman microscope. The reduced acquisition time and specificity of the signal allows for a quantitative and real-time characterization of surface species, hitherto not possible using Raman spectroscopy. NWERS provides a direct analytic tool for monolayer research and also opens a route to compact microscopeless lab-on-a-chip devices with integrated sources, spectrometers, and detectors fabricated using a mass-producible complementary metal oxide semiconductor technology platform.