The BMP-related protein Radar:: a maintenance factor for dorsal neuroectoderm cells?
The BMP-related protein Radar:: a maintenance factor for dorsal neuroectoderm cells?
复制标题
DOI：
10.1016/s0925-4773(99)00026-x
复制
发表时间：
1999-07-01
影响因子：
2.6
通讯作者：
Rosa, FM
中科院分区：
文献类型：
--
作者：
Délot, E;Kataoka, H;Rosa, FM
我们以前已经克隆了几个成员的TGF-β超家族的生长因子在斑马鱼，其中之一，雷达，属于Dpp-Vg 1相关（DVR）的亚组，具有最高的同源性GDF 6。雷达的表达模式表明可能参与胚胎发育过程中的几个诱导步骤，包括背神经管，红细胞，背鳍和视网膜。我们已经分析了雷达的表达模式与背神经管结构的标记物，msxC的比较，并显示雷达和msxC在整个胚胎发生中在相似和/或相邻的组织中表达。为了证明这两种蛋白质之间的功能关系，我们已经产生了雷达的全长cDNA，并表明通过DNA注射的雷达过表达在组织中保持msxC的表达，在该组织中，它通常表达然后关闭，特别是在背侧神经外胚层中。携带雷达缺失的突变体的表型的研究显示背神经管的细胞的身份和死亡的损失。综上所述，这些结果表明，种族可能参与维持身份的细胞的背侧最神经管和至少一个子集的神经嵴细胞。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
We have previously cloned several members of the TGF-beta superfamily of growth factors in zebrafish, one of which, Radar, belongs to the Dpp-Vg1-related (DVR) subgroup, with highest homology to GDF6. The pattern of expression of Radar suggested a possible involvement in several induction steps during embryogenesis including in the dorsal neural tube, red blood cells, the dorsal fin and the retina. We have analyzed the pattern of expression of Radar in comparison with that of a marker of dorsal neural tube structures, msxC and show that Radar and msxC are expressed in similar and/or adjacent tissues throughout embryogenesis. In order to demonstrate a functional relationship between these two proteins, we have generated a full-length cDNA for Radar and shown that Radar overexpression by DNA injection maintains expression of msxC in tissues where it is normally expressed then turned off, in particular in the dorsal neurectoderm. Study of the phenotype of a mutant carrying a deletion of Radar shows a loss of identity and death of the cells of the dorsal neural tube. Taken together these results suggest that Racial could be involved in maintaining the identity of cells of the dorsal-most neural tube and of at least a subset of neural crest cells. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.