Permanganate oxidation reactions of DNA: Perspective in biological studies
Permanganate oxidation reactions of DNA: Perspective in biological studies
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DOI：
10.1081/ncn-120023276
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发表时间：
2003-01-01
影响因子：
1.3
通讯作者：
Cotton, RGH
中科院分区：
文献类型：
--
作者：
Bui, CT;Rees, K;Cotton, RGH
KMnO4作为一种强大的化学探针在生物领域有着广泛的应用，特别是在DNA构象研究中的应用。KMnO4分析为了解生化过程和检测与许多遗传性疾病相关的异常DNA提供了必要的信息。典型的例子有测序技术、转录研究的足迹分析、激素受体结合分析的干扰方法以及Z-DNA、Z-Z连接、发夹、曲率、短核苷酸碱基重复、嵌入物和沟槽结合的DNA构象研究等。最近，KMnO4已成功地用于检测DNA的单碱基变化和突变(化学错配法，CCM)以及其他类型的碱基损伤(8-氧鸟嘌呤和胸腺嘧啶二聚体)。本文旨在综述高锰酸盐氧化反应在DNA的各种生物学研究中的作用和局限性。
KMnO4 has been well known as a powerful chemical probe for numerous applications in biological fields, particularly for those used in conformational studies of DNA. The KMnO4 assay provides essential information for understanding biochemical processes and detecting aberrant DNA, which is associated with many genetic diseases. Elegant examples are sequencing techniques, foot-printing assays for transcriptional studies, an interference method for hormone receptor binding assays as well as DNA conformational studies of Z-DNA, Z-Z junctions, hairpins, curvatures, short nucleotide base repeats, binding of intercalators and groove binders, etc. Recently, KMnO4 has been successfully applied to detect single base changes and mutations in DNA (chemical cleavage of mismatch method, CCM) as well as other types of base damage (8-oxoguanine and thymine dimers). This paper aims to review the usefulness and limitations of the permanganate oxidation reaction used in various biological studies of DNA.