Role of Tobacco vein banding mosaic virus 3 '-UTR on virus systemic infection in tobacco
Role of Tobacco vein banding mosaic virus 3 '-UTR on virus systemic infection in tobacco
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DOI：
10.1016/j.virol.2018.11.001
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
Li Xiang Dong
中科院分区：
文献类型：
--
作者：
Wang Ying;Yang Zheng You;Tian Yan Ping;Geng Chao;Yuan Xue Feng;Li Xiang Dong
为探讨烟草脉带花叶病毒(TVBMV)3‘端非编码区在病毒系统侵染中的作用，将3种类型的缺失引入TVBMV侵染性克隆pCaTVBMV-GFP中。3‘-非编码区8-42、43-141或163-174位核苷酸缺失的突变株未引起全身性感染。底栖植物。其他缺失突变导致延迟的全身性感染和较温和的静脉清除和花叶症状。大多数后代突变病毒在宿主植物中经过一次传代后，都获得了与缺失的核苷酸序列相似或不同的核苷酸。TVBMV 3‘-非编码区5’末端8-42位核苷酸可形成类茎环结构，对烟草TVBMV系统运动起关键作用。我们认为，这种类似SL的结构，从而3‘-UTR，在TVBMV系统感染中起着重要作用。
To investigate the role ofTobacco vein banding mosaic virus(TVBMV) 3′-UTR in virus systemic infection, three types of deletions were introduced into TVBMV infectious clone pCaTVBMV-GFP. Mutants with deletions at the nucleotide position 8–42, 43–141, or 163–174 in the 3'-UTR failed to cause systemic infection inN. benthamianaplants. Other deletion mutants caused delayed systemic infection and milder vein clearing and mosaic symptoms. Most progeny mutant virus had acquired nucleotides, similar to or different from the deleted nucleotide sequences, after a single passage in the host plant. Nucleotides at the position 8–42 near the 5′-terminus of TVBMV 3′-UTR could form a stem-loop (SL) like structure which was crucial for TVBMV systemic movement in tobacco. We proposed that this SL like structure, and thus 3′-UTR, has an essential role in TVBMV systemic infection.