An in vitro diffusion model for the study of calcification of bovine pericardium tissue.
An in vitro diffusion model for the study of calcification of bovine pericardium tissue.
复制标题
用于研究牛心包组织钙化的体外扩散模型。
DOI：
10.1021/js970049b
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Shapiro,AJ
中科院分区：
文献类型：
--
作者：
Tomazic,BB;Chow,LC;Carey,CM;Shapiro,AJ
Bovine pericardium (BP) is extensively used for the production of heart valve bioprostheses. BP has excellent mechanical properties but a limited lifespan because of intrinsic subsurface calcification in vivo. In this study, the in vitro mineralization of BP was investigated by a novel diffusion cell model. In two sets of experiments, glutaraldehyde‐treated BP membranes were placed between two compartments, both of which contained calcium phosphate solutions made by equilibration of octacal‐cium phosphate (Exp I) or dicalcium phosphate dihydrate (Exp II) in phosphoric acid. The movement of calcium (Ca), phosphate (P), and protons through the BP membrane was followed throughout the diffusion process. Histology, scanning electron microscopy, wet chemical analysis, and energy dispersive X‐ray analyses provided good evidence of subsurface mineralization of BP that resembled in vivo mineral deposition. Energy dispersive x‐ray microanalyses found a Ca/P heterogeneity of the early subsurface mineral that formed in the membrane. The use of a diffusion cell to model BP calcification under well‐characterized conditions has led to in vitro mineralization that more closely matches that observed in vivo. The results suggest that this in vitro diffusion model can be used to study the mechanism of pathological mineralization. This model has the potential to provide rapid, inexpensive, basic information about the mineralization process.
