Voltage-dependent calcium channels from Paramecium cilia incorporated into planar lipid bilayers.
Voltage-dependent calcium channels from Paramecium cilia incorporated into planar lipid bilayers.
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来自草履虫纤毛的电压依赖性钙通道并入平面脂质双层。
DOI：
10.1126/science.6330895
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Kung,C
中科院分区：
文献类型：
--
作者：
Ehrlich,BE;Finkelstein,A;Forte,M;Kung,C
两种不同的二价阳离子选择性通道草履虫纤毛纳入平面脂质双层。这两个渠道更容易渗透二价比单价阳离子，其中一个显着的二价阳离子之间的歧视。后者通道的选择性和电压依赖性与在各种细胞中发现的电压依赖性钙通道的选择性和电压依赖性相当。钙通道的生物化学、生物物理学和遗传学的综合研究现在成为可能。
Two different divalent cation-selective channels from Paramecium cilia were incorporated into planar lipid bilayers. Both channels were much more permeable to divalent than univalent cations, and one of them discriminated significantly among the divalent cations. The selectivity and voltage dependence of the latter channel are comparable to those of voltage-dependent calcium channels found in a variety of cells. A combined biochemical, biophysical, and genetic study of calcium channels is now possible.