Dietary cod-liver oil improves endothelium-dependent responses in hypercholesterolemic and atherosclerotic porcine coronary arteries.
Dietary cod-liver oil improves endothelium-dependent responses in hypercholesterolemic and atherosclerotic porcine coronary arteries.
复制标题
膳食鱼肝油可改善高胆固醇血症和动脉粥样硬化猪冠状动脉的内皮依赖性反应。
DOI：
10.1161/01.cir.78.6.1421
复制
发表时间：
1988
期刊：
影响因子：
37.8
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
--
作者：
Shimokawa,H;Vanhoutte,PM
本研究探讨了饮食中补充鱼肝油对高胆固醇血症和猪冠状动脉粥样硬化中内皮依赖性舒张功能受损的影响。对16只雄性约克郡猪进行左冠状动脉球囊内皮移除，并喂食2%高胆固醇饮食10周，饮食中补充或不补充鱼肝油（30 ml/天）（分别为油喂食组和胆固醇喂食组）。该模型允许同时检查饮食鱼肝油对高胆固醇血症（右冠状动脉）和动脉粥样硬化（左冠状动脉）血管反应性的影响。喂养10周后，用鱼肝油进行饮食治疗引起血浆二十碳五烯酸水平增加和血浆花生四烯酸水平降低，而治疗对高胆固醇喂养引起的血浆脂质水平增加没有显著影响。形态计量学分析显示，治疗对冠状动脉粥样硬化有显著抑制作用。内皮依赖性反应进行了检查，在体外环的准备工作和生物测定实验。与胆固醇喂养的动物相比，在油喂养的动物的右和左冠状动脉中，缓激肽、5-羟色胺和5 '-二磷酸腺苷的内皮依赖性舒张作用更大。从胆固醇喂养和油喂养的猪聚集血小板诱导可比的，更大的内皮依赖性舒张环从油喂养比从胆固醇喂养的猪。由5-羟色胺或聚集的血小板引起的收缩在有内皮的油饲猪的环中被显著抑制，而在两组中没有内皮的环中它们相当。硝普钠的舒张和氯化钾或5-羟色胺的收缩在两组无内皮的环中相当。生物测定实验表明，释放内皮源性舒张因子响应缓激肽和血管平滑肌舒张内皮因子后鱼油饮食更大。这些结果表明，饮食补充鱼肝油延迟损伤的内皮依赖性舒张在高胆固醇血症和动脉粥样硬化，部分原因是改善释放的内皮源性舒张因子，部分原因是改善舒张冠状动脉平滑肌的因素。
This study examined the effects of dietary supplementation with cod-liver oil on impaired endothelium-dependent relaxations in hypercholesterolemia and in atherosclerosis in porcine coronary arteries. Sixteen male Yorkshire pigs underwent balloon endothelium removal of the left coronary arteries and were fed a 2% high-cholesterol diet for 10 weeks, with or without dietary supplementation of cod-liver oil (30 ml/day) (oil-fed and cholesterol-fed groups, respectively). This model allowed the simultaneous examination of the effects of dietary cod-liver oil on vascular reactivity in hypercholesterolemia alone (right coronary artery) and in atherosclerosis (left coronary artery). After 10 weeks of feeding, the dietary treatment with cod-liver oil caused an increase in plasma levels of eicosapentaenoic acid and a decrease in the plasma levels of arachidonic acid, whereas the treatment had no significant effect on the increases in plasma lipid levels induced by the high-cholesterol feeding. Morphometric analysis showed significant inhibition of coronary atherosclerosis by the treatment. Endothelium-dependent responses were examined in vitro in ring preparations and in bioassay experiments. Endothelium-dependent relaxations to bradykinin, serotonin, and adenosine 5'-diphosphate were larger in both right and left coronary arteries from oil-fed than from cholesterol-fed animals. Aggregating platelets from cholesterol-fed and oil-fed pigs induced comparable, larger endothelium-dependent relaxations in rings from oil-fed than from cholesterol-fed pigs. The contractions induced by serotonin or aggregating platelets were significantly inhibited in rings with endothelium from oil-fed pigs, whereas they were comparable in rings without endothelium in both groups. Relaxations to sodium nitroprusside and contractions to potassium chloride or serotonin were comparable in rings without endothelium in both groups. The bioassay experiments revealed that the release of endothelium-derived relaxing factor in response to bradykinin and the relaxations of vascular smooth muscle to the endothelial factor were greater after the fish-oil diet. These results indicate that dietary supplementation of cod-liver oil delays the impairment of endothelium-dependent relaxations in hypercholesterolemia and in atherosclerosis, partly because of an improved release of endothelium-derived relaxing factor and partly because of an improved relaxation of coronary smooth muscle to the factor.