Adhesive properties and antibiotic resistance of Klebsiella, Enterobacter, and Serratia clinical isolates involved in nosocomial infections
Adhesive properties and antibiotic resistance of Klebsiella, Enterobacter, and Serratia clinical isolates involved in nosocomial infections
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DOI：
10.1128/jcm.34.8.1963-1969.1996
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发表时间：
1996-08-01
影响因子：
9.4
通讯作者：
Joly, B
中科院分区：
文献类型：
--
作者：
Livrelli, V;DeChamps, C;Joly, B
克雷伯氏菌属、肠杆菌属和沙雷氏菌属（KES）菌株在肠道定植是医院感染发展的关键步骤。我们研究了103株KES临床分离株的粘附特性、抗生素耐药性和参与定植或感染的情况：30株肺炎克雷伯菌（29%）、16株产酸克雷伯菌（15%）、30株产气肠杆菌（29%）、14株阴沟肠杆菌（14%）和13株沙雷氏菌属（13%）分离株。其中一半对几种抗菌药物耐药，包括氨基糖苷类和β-内酰胺类抗生素。总共27个30 K。pneumoniae分离株（90%）与人细胞株Intestine-407（Int-407）有粘附作用，而K. pneumoniae分离株与人细胞株Intestine-407（Int-407）无粘附。oxytoca或E.产气荚膜梭菌和大肠杆菌中只有2株粘附。pnenmoniae：57%的菌株为聚集粘附，1个菌株为扩散粘附，30%的菌株为新的粘附模式--局部粘附。产生CAZ-5 β-内酰胺酶的肺炎分离株。此外，45%的局部粘附菌株与严重感染有关。用特异性DNA探针检测了cf29、pap和afa/Dr粘附素编码基因，发现粘附模式与特异性菌毛的产生无关，提示可能涉及几种尚未被认识的粘附因子。我们的研究表明，与抗菌药物耐药性相关的特殊粘附特性可以解释某些医院菌株的致病性。
Intestinal colonization by Klebsiella, Enterobacter, and Serratia (KES) strains is a crucial step in the development of nosocomial infections. We studied the adhesive properties, antibiotic resistance, and involvement in colonization or infection of 103 KES clinical isolates: 30 Klebsiella pneumoniae (29%), 16 Klebsiella oxytoca (15%), 30 Enterobacter aerogenes (29%), 14 Enterobacter cloacae (14%), and 13 Serratia sp. (13%) isolates. Half of them were resistant to several antimicrobial agents, including aminoglycosides and beta-lactam antibiotics. A total of 27 of 30 K. pneumoniae isolates (90%) adhered to the human cell line Intestine-407 (Int-407), while none of the K. oxytoca or E. aerogenes isolates and only 2 of the E, cloacae isolates adhered, Three adhesive patterns were observed for K. pnenmoniae: an aggregative adhesion in 57% of the isolates, a diffuse adhesion in only one isolate, and a new pattern, localized adhesion, in 30% of the isolates, While most of the sensitive strains adhered with the aggregative phenotype, the localized pattern was associated with resistant K. pneumoniae isolates producing the CAZ-5 beta-lactamase. Furthermore, 45% of such localized-adhesion isolates were involved in severe infections, The distributions of type 1 and type 3 fimbriae, enteroaggregative E. coli, and cf29, pap, and afa/Dr adhesin-encoding genes were determined by using specific DNA probes, No relationship was found between the adhesive pattern and the production of specific fimbriae, suggesting that several unrecognized adhesive factors are involved. Our study indicates that special adhesive properties associated with resistance to antimicrobial agents could account for the pathogenicity of certain nosocomial strains.