Study of kernel structure of high-amylose and wild-type rice by X-ray microtomography and SEM
Study of kernel structure of high-amylose and wild-type rice by X-ray microtomography and SEM
复制标题
X射线显微断层扫描和扫描电镜研究高直链淀粉和野生型水稻籽粒结构
DOI：
10.1016/j.jcs.2011.08.013
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发表时间：
2012-01-01
影响因子：
3.8
通讯作者：
Shi, Yong-Cheng
中科院分区：
文献类型：
--
作者：
Zhu, Li-Jia;Dogan, Hulya;Shi, Yong-Cheng
利用高分辨X射线显微断层扫描和环境扫描电子显微镜对转基因高直链淀粉水稻品系及其野生型的稻米进行了研究。二维（2-D）的横截面图像和三维物体从XMT重建和分析，以阐明其结构特征。高直链淀粉水稻（HAR）缺乏淀粉分支酶的两种同工酶，称为SBEI和SBEIIb，导致了与野生型水稻（WTR）明显不同的籽粒内部密度。HAR具有较小的细长淀粉粒，在籽粒内具有空气空间，导致不透明的籽粒，而WTR具有紧密的胚乳，几乎没有空气空间和多边形淀粉粒。XMT允许一个完整的3-D表征的水稻籽粒结构，并揭示了空气空间分布是不均匀的HAR内核。(C)2011爱思唯尔有限公司保留所有权利。
Rice kernels of a transgenic high-amylose rice line and its wild type were examined by a high-resolution X-ray microtomography (XMT) and an environmental scanning electron microscope. Two-dimensional (2-D) cross-sectional images and 3-D objects from XMT were reconstructed and analyzed to elucidate their structural features. The lack of two isoforms of starch branching enzyme, termed SBEI and SBEIIb in high-amylose rice (HAR), resulted in a distinctly different grain inner density than wild-type rice (WTR). HAR had smaller, elongated starch granules with air spaces inside the kernels resulting in an opaque grain, whereas WTR had a tight endosperm with little air space and polygonal starch granules. XMT allowed a full 3-D characterization of the rice kernel structure and revealed that air space distribution was not uniform in the HAR kernel. (C) 2011 Elsevier Ltd. All rights reserved.