The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production
The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production
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DOI：
10.1016/s1074-7613(00)80464-1
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发表时间：
1998-01-01
期刊：
影响因子：
32.4
通讯作者：
Mak, TW
中科院分区：
文献类型：
--
作者：
Yoshida, H;Nishina, H;Mak, TW
NF-ATc1 是编码活化 T 细胞核因子 (NF-AT) 细胞质成分的基因家族的成员。在活化的 T 细胞中，核 NF-AT 与多个细胞因子基因的启动子区域结合并诱导其转录。使用纯合 NF-ATc1(-/-) 突变型 ES 细胞系在重组激活基因 1 (RAG-1) 缺陷型囊胚互补测定中研究 NF-ATc1 的作用。 NF-ATc1(-/-)/RAG-1(-/-)嵌合小鼠胸腺细胞数量减少，外周淋巴细胞增殖受损，但IL-2产生正常。 IL-4 和 IL-6 产生减少表明，突变 T 细胞的体外 Th2 反应诱导受到损害。这些数据表明 NF-ATc1 在 T 淋巴细胞的发育和 Th2 反应的分化中发挥作用。
NF-ATc1 is a member of a family of genes that encodes the cytoplasmic component of the nuclear factor of activated T cells (NF-AT). In activated T cells, nuclear NF-AT binds to the promoter regions of multiple cytokine genes and induces their transcription. The role of NF-ATc1 was investigated in recombination activating gene-1 (RAG-1)-deficient blastocyst complementation assays using homozygous NF-ATc1(-/-) mutant ES cell lines. NF-ATc1(-/-)/RAG-1(-/-) chimeric mice showed reduced numbers of thymocytes and impaired proliferation of peripheral lymphocytes, but normal production of IL-2. Induction in vitro of Th2 responses, as demonstrated by a decrease in IL-4 and IL-6 production, was impaired in mutant T cells. These data indicate that NF-ATc1 plays roles in the development of T lymphocytes and in the differentiation of the Th2 response.