Adjuvant activity in delayed hypersensitivity of the peptidic part of bacterial peptidoglycans
Adjuvant activity in delayed hypersensitivity of the peptidic part of bacterial peptidoglycans
复制标题
细菌肽聚糖肽部分迟发型超敏反应的佐剂活性
作者：
J. Fleck;M. Mock;F. Tytgat;C. Nauciel;R. Minck
人们普遍认为，弗氏完全佐剂中存在灭活的分枝杆菌对于诱导对可溶性抗原的典型迟发型超敏反应是必要的，并且已经尝试分离活性成分。最初获得的制剂具有肽糖脂性质2。含有阿拉伯半乳聚糖和壁肽聚糖的水溶性佐剂已被分离出来3-5。我们之前已经证明，几种革兰氏阴性细菌的纯化细胞壁肽聚糖在诱导迟发型超敏反应方面表现出与分枝杆菌相同的佐剂作用6, 7，并且二糖肽单体（β-1, 4-N-乙酰葡糖胺基-N-乙酰胞壁酰-l-二氨基庚二酸（l）-d-Ala）仍然具有活性8。对分枝杆菌9、10的肽聚糖也进行了类似的独立研究，其亚基结构仅略有不同：N-乙酰胞壁酸被N-乙醇酰胞壁酸取代，并且Glu和二氨基庚二酸残基均具有酰胺取代基。本研究旨在确定肽聚糖佐剂活性与何种化学结构相关。
IT is generally accepted that the presence of killed mycobacteria in complete Freund's adjuvant is necessary to induce a typical delayed hypersensitivity to soluble antigens1 and attempts have been made to isolate the active component. The preparations obtained initially were of a peptidoglycolipidic nature2. Hydrosoluble adjuvants containing an arabinogalactan and the wall peptidoglycan have been isolated3–5. We have shown previously that the purified cell wall peptidoglycan of several Gram-negative bacteria exhibit the same adjuvant effect as mycobacteria for the induction of delayed hypersensitivity6, 7 and that the disaccharide peptide monomer (β-1, 4-N-acetylglucosaminyl-N-acetylmuramyl-l-diaminopimelic acid (l)-d-Ala) was still active8. Similar findings have been made independently with the peptidoglycan of mycobacteria9, 10, the subunit structure of which is only slightly different: N-acetylmuramic acid is replaced by N-glycolylmuramic acid and both Glu and diaminopimelic acid residues have amide substituents. The present study was undertaken to determine to what chemical structure of the peptidoglycan adjuvant activity was related.