NARROW LEAF 7 controls leaf shape mediated by auxin in rice
NARROW LEAF 7 controls leaf shape mediated by auxin in rice
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DOI：
10.1007/s00438-008-0328-3
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发表时间：
2008-05-01
影响因子：
3.1
通讯作者：
Sekiguchi, Hiroshi
中科院分区：
文献类型：
--
作者：
Fujino, Kenji;Matsuda, Yasuyuki;Sekiguchi, Hiroshi
阐明控制叶片形状的遗传基础可用于操纵作物性状，从而使作物产量更加稳定和增加。为了加深我们对控制叶片形状过程的理解，我们在水稻中发现了一个突变基因，该基因会导致叶片宽度显着减小，称为窄叶 7 (nal7)。 nal7 的这种自发突变发生在水稻品种与野生型叶表型杂交产生的高级回交后代的过程中。虽然突变导致叶片宽度减小，但除了泡状细胞外，在叶片细胞水平上没有观察到显着的形态变化。 NAL7 基因座编码一种含黄素的单加氧酶，与 YUCCA 显示序列同源性。对 NAL7 结构模型的检查表明该突变导致酶失活。与野生型相比，nal7突变体中的IAA含量发生了改变。过表达 NAL7 cDNA 的 nal7 突变体表现出根的过度生长和异常形态，这可能是由于生长素过量产生。这些结果表明NAL7参与生长素生物合成。
Elucidation of the genetic basis of the control of leaf shape could be of use in the manipulation of crop traits, leading to more stable and increased crop production. To improve our understanding of the process controlling leaf shape, we identified a mutant gene in rice that causes a significant decrease in the width of the leaf blade, termed narrow leaf 7 (nal7). This spontaneous mutation of nal7 occurred during the process of developing advanced backcrossed progeny derived from crosses of rice varieties with wild type leaf phenotype. While the mutation resulted in reduced leaf width, no significant morphological changes at the cellular level in leaves were observed, except in bulliform cells. The NAL7 locus encodes a flavin-containing monooxygenase, which displays sequence homology with YUCCA. Inspection of a structural model of NAL7 suggests that the mutation results in an inactive enzyme. The IAA content in the nal7 mutant was altered compared with that of wild type. The nal7 mutant overexpressing NAL7 cDNA exhibited overgrowth and abnormal morphology of the root, which was likely to be due to auxin overproduction. These results indicate that NAL7 is involved in auxin biosynthesis.