The occurrence of two types of mitochondrial DNA in rat populations as detected by EcoRI endonuclease analysis
The occurrence of two types of mitochondrial DNA in rat populations as detected by EcoRI endonuclease analysis
复制标题
通过 EcoRI 核酸内切酶分析检测大鼠群体中两种类型线粒体 DNA 的出现
DOI：
10.1016/0014-5793(77)80805-3
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发表时间：
1977
期刊：
影响因子：
3.5
通讯作者：
M. Simpson
中科院分区：
文献类型：
--
作者：
James F. Francisco;M. Simpson
最近，对许多哺乳动物物种的线粒体DNA（mtDNA）的限制性内切酶研究表明，一种以上的mtDNA可以出现在一个物种中[1]。当用Hael II内切酶检测时，包括大鼠在内的12个不同物种只显示出单一类型的mtDNA，而研究的10匹马中的每一匹都产生了5种不同模式中的一种，每个人类来源都产生了不同的模式。因此，这些结果和最近两项研究的结果，其中使用了几种限制性内切酶，包括EcoRI，并没有表明在大鼠种群中存在一种以上的mtDNA [2，3]。我们对'8 S' mtDNA起源的研究[4，5]导致了对从Sprague道利大鼠合并肝脏中获得的mtDNA的EcoRI分析。所获得的凝胶模式表明存在两个完整尺寸的mtDNA环。将这些研究扩展到个体肝脏和另外两个大鼠品系表明，虽然个体大鼠肝脏似乎含有单一类型的mtDNA，但存在两种类型的mtDNA，这两种类型的mtDNA都分布在所研究的每个大鼠品系中。
Recently, a restriction enzyme study of the mitochondrial DNA (mtDNA) of a number of mammalian species showed that more than one type of mtDNA can occur in a single species [1]. While twelve diverse species, including the rat, showed only a single type of mtDNA when examined with HaelII endonuclease, each of the ten horses studied yielded one of five different patterns, and each human source yielded a distinct pattern. Thus, these results and the results of two more recent studies, in which several restriction endonucleases, including EcoRI, were used, have not indicated the occurrence of more than one type of mtDNA in the rat population [2, 3]. Our investigations into the origins of'8 S'mtDNA [4, 5] led to the EcoRI analysis of mtDNA obtained from the pooled livers of Sprague Dawley rats. The gel patterns obtained suggested the presence of two full size mtDNA circles. Extension of these studies to individual livers and to two additional rat strains showed that, while the individual rat liver appears to contain a single type of mtDNA, there occur two types of mtDNA both of which are distributed throughout each rat strain examined.