Changes in matrix metalloproteinase 2 activities in amniochorions during premature rupture of membranes
Changes in matrix metalloproteinase 2 activities in amniochorions during premature rupture of membranes
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DOI：
10.1016/j.jsgi.2006.10.001
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发表时间：
2006-12-01
期刊：
影响因子：
--
通讯作者：
Nagaoka, Isao
中科院分区：
文献类型：
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作者：
Ota, Atsuyuki;Yonemoto, Hisashi;Nagaoka, Isao
目的：基质金属蛋白酶 (MMP)（例如 MMP-3 和 MMP-9）的蛋白水解活性增加与足月胎膜早破相关。然而，MMP-2是否参与胎膜早破尚不清楚。在本研究中，为了阐明 MMP-2 的作用，我们评估了 MMP-2 的活性以及 MMP-2 前体、膜型 1 (MT1)-MMP 和金属蛋白酶组织抑制剂 (TIMP)-1 在胎膜早破中的表达。方法：从 29 名未临产（剖宫产；CS，n = 10）、临产（正常分娩；AD，n =）的受试者中制备羊绒毛膜。 10），以及分娩期间胎膜早破（PROM，n = 9）。通过测量 MMP-2 特异性底物的消化情况，采用分光光度法测定 MMP-2 活性。 Western免疫印迹法检测pro-MMP-2、MT1-MMP和TIMP-1的水平。结果：PROM中MMP-2活性显着高于CS和ND（P
OBJECTIVE: Increased proteolytic activities of matrix metalloproteinases (MMPs) such as MMP-3 and MMP-9 are associated with premature rupture of membranes at term. However, it is unclear whether MMP-2 is involved in the premature rupture of membranes. In this study, to elucidate the role of MMP-2, we evaluated the activity of MMP-2 and also the expression of pro-MMP-2, membrane type 1 (MT1)-MMP and tissue inhibitor of metalloproteinase (TIMP)-1 in premature rupture of membranes.METHODS: Amniochorions were prepared from 29 subjects with no labor (cesarean section; CS, n = 10), labor (normal delivery; AD, n = 10), and labor during, premature rupture of membranes (PROM, n = 9). MMP-2 activity was spectrophotometrically assayed by measuring the digestion of an MMP-2-specific substrate. The levels of pro-MMP-2, MT1-MMP and TIMP-1 were determined by Western immunoblotting.RESULTS: The activity of MMP-2 in PROM was significantly higher than that in CS and ND (P