Proliferation of Haemopoietic Stem Cells in Vitro
Proliferation of Haemopoietic Stem Cells in Vitro
复制标题
造血干细胞的体外增殖
DOI：
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发表时间：
1974
影响因子：
6.5
通讯作者：
L. Lajtha
中科院分区：
文献类型：
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作者：
T. Dexter;L. Lajtha
总结。描述了一种小鼠造血细胞的液体培养系统，其中粒细胞（G -培养）或单核细胞（M -培养）的增殖可以维持数周。在M -培养中，脾脏集落形成细胞（cfu）迅速丢失。琼脂集落形成细胞（CFUc）存在6周，然后迅速下降。由这些CFUc形成的菌落只含有大的单核细胞。在G‐培养中，cfu增殖，其移植物（培养7‐9周后）保护致命辐射小鼠免于造血死亡。同样，在分化的所有阶段，CFUc都有增殖，在由粒细胞组成的琼脂中形成菌落。
Summary. A liquid culture system for murine haemopoietic cells is described where proliferation of granulocytes (G‐cultures) or mononuclear cells (M‐cultures) can be maintained for many weeks. In M‐cultures there is a rapid loss of spleen colony forming cells (CFUs). Agar colony forming cells (CFUc) are present for 6 weeks, then decline rapidly. Colonies formed from these CFUc contain only large mononuclear cells. In G‐cultures there is proliferation of CFUs, grafts of which (after 7‐9 weeks in culture) protect lethally irradiated mice from haemopoietic death. Similarly, there is proliferation of CFUc, which form colonies in agar consisting of granulocytes, in all stages of differentiation.