Establishment of Coral-Bacteria Symbioses Reveal Changes in the Core Bacterial Community With Host Ontogeny
Establishment of Coral-Bacteria Symbioses Reveal Changes in the Core Bacterial Community With Host Ontogeny
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DOI：
10.3389/fmicb.2019.01529
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发表时间：
2019-07-09
影响因子：
5.2
通讯作者：
Huggett, Megan J.
中科院分区：
文献类型：
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作者：
Bernasconi, Rachele;Stat, Michael;Huggett, Megan J.
细菌群落是珊瑚的基本共生体。然而，细菌群落在珊瑚的整个生命史中获得的过程，特别是在幼虫和幼年早期阶段，仍然知之甚少。在这里，分析了石珊瑚的细菌从成虫到产卵精子束的转移，以及在早期发育阶段（幼虫和新定居的卵）和 6 个月大的幼虫的获得。幼虫在受控环境条件下饲养，利用 16S rRNA 基因的扩增子测序来确定可能与宿主建立共生关系的细菌来源（母体、海水或沉积物）。母体菌落直接将细菌从红杆菌科、冷菌科和内生菌科转移到卵子束中。此外，各代之间的微生物群落结构存在显着差异，但早期生命史阶段的珊瑚细菌群落结构并未受到不同环境饲养条件的影响。这些数据表明，细菌群落的吸收和结构是受发育调节的，而不是环境调节的。母体珊瑚菌落和环境基质中普遍存在的细菌都是共生体的潜在来源，对于建立珊瑚微生物组至关重要。独特的是，我们报告了核心细菌群落和常驻细菌群落的个体发育存在变异，支持了微生物群落可能在珊瑚宿主的不同生活史阶段中发挥特定作用的假设。
Bacterial communities are fundamental symbionts of corals. However, the process by which bacterial communities are acquired across the life history of corals, particularly in larval and early juvenile stages, is still poorly characterized. Here, transfer of bacteria of the Scleractinian coral Acropora digitifera from adults to spawned egg-sperm bundles was analyzed, as well as acquisition across early developmental stages (larvae and newly settled spat), and 6-month-old juveniles. Larvae were reared under manipulated environmental conditions to determine the source (maternal, seawater, or sediment) of bacteria likely to establish symbiotic relationships with the host using amplicon sequencing of the 16S rRNA gene. Maternal colonies directly transferred bacteria from the families Rhodobacteraceae, Cryomorphaceae, and Endozoicimonaceae to eggsperm bundles. Furthermore, significant differences in the microbial community structure were identified across generations, yet the structure of the coral bacterial community across early life history stages was not impacted by different environmental rearing conditions. These data indicate that the uptake and structure of bacterial communities is developmentally, rather than environmentally, regulated. Both maternal coral colonies and ubiquitous bacteria found across environmental substrates represent a potential source of symbionts important in establishing the coral microbiome. Uniquely, we report the presence of variation with ontogeny of both the core and resident bacterial communities, supporting the hypothesis that microbial communities are likely to play specific roles within the distinct life history stages of the coral host.