Antibacterial activity and mechanism of linalool against Pseudomonas aeruginosa
Antibacterial activity and mechanism of linalool against Pseudomonas aeruginosa
复制标题
芳樟醇对铜绿假单胞菌的抗菌活性及机制
DOI：
10.1016/j.micpath.2020.103980
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发表时间：
2020-04-01
影响因子：
3.8
通讯作者：
Chen, Wenxue
中科院分区：
文献类型：
--
作者：
Liu, Xue;Cai, Jiaxin;Chen, Wenxue
本研究旨在评价芳樟醇对铜绿假单胞菌的抑菌作用及其机制。根据最低抑菌浓度(MIC)和最低杀菌剂浓度(MBC)测定其抗菌活性。通过生长曲线测定、扫描电子显微镜、细胞膜通透性、膜电位和呼吸链脱氢酶测定等方法，探讨了其抗菌机理。芳樟醇的最低抑菌浓度和最小杀菌浓度分别为431和862微克/毫升。生长曲线分析表明，铜绿假单胞菌的生长受到抑制。扫描电子显微镜结果显示，芳樟醇破坏了细胞的正常形态。核酸的释放和膜电位的降低证明铜绿假单胞菌的膜完整性被破坏。呼吸链脱氢酶检测表明，随着490 nm吸光度的降低，呼吸链发生损伤。这项研究表明，芳樟醇有可能通过破坏细胞膜，导致细胞死亡而成为食物的防腐剂。
The purpose of this study was to evaluate the antibacterial activity and mechanism of linalool against Pseudomonas aeruginosa. The determination of antibacterial activity was based on the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC). Further, the antibacterial mechanism was explored by a growth curve assay, scanning electron microscopy (SEM), cell membrane permeability, membrane potential and respiratory chain dehydrogenase determination. The MIC and the MBC of linalool were 431 mu g/mL and 862 mu g/mL, respectively. The growth curve assay showed that the growth of P. aeruginosa was inhibited. The results of SEM revealed that linalool disrupted the normal morphology of the cell. The release of nucleic acids as well as the decrease in the membrane potential proved that the membrane integrity of P. aeruginosa was destroyed. Moreover, the respiratory chain was damaged by respiratory chain dehydrogenase determination as the absorbance at 490 nm decreased. This research suggested that it was possible for linalool to become a preservative of food by destroying the cell membrane, resulting in cell death.