Developmental stages and gut microenvironments influence gut microbiota dynamics in the invasive beetle Popillia japonica Newman (Coleoptera: Scarabaeidae)
Developmental stages and gut microenvironments influence gut microbiota dynamics in the invasive beetle Popillia japonica Newman (Coleoptera: Scarabaeidae)
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DOI：
10.1111/1462-2920.14797
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发表时间：
2019-10-21
影响因子：
5.1
通讯作者：
Montagna, Matteo
中科院分区：
文献类型：
--
作者：
Chouaia, Bessem;Goda, Nizar;Montagna, Matteo
日本金龟子（Popilliajaponica纽曼）（鞘翅目：金龟子科）是一种多食性入侵甲虫，原产于日本。这种昆虫具有很强的适应力，能够迅速适应新的植被。昆虫相关微生物在昆虫生理学中发挥重要作用，帮助其宿主适应不断变化的条件，并可能有助于昆虫的入侵潜力。这种共生细菌可以是核心微生物群的一部分，其在宿主的整个生命周期中稳定地传播，或者在每个发育阶段从环境中选择性地招募。本研究的目的是调查与来自意大利的日本对虾入侵种群相关的细菌群落的起源、稳定性和周转。我们的研究结果表明，土壤微生物代表了肠道细菌的重要来源，但随着昆虫的发展，其肠道微生物群的丰富性和多样性大幅下降，群落组成的变化。值得注意的是，只有16.75%的土壤细菌存在于幼虫中，直到成虫阶段。我们进一步确定了不同肠道部分的微环境是影响该物种微生物群组成的重要因素，这可能是由于pH值，氧气可用性和氧化还原电位的差异。此外，P. japonica还在所有发育阶段都具有稳定的细菌群落，由众所周知的降解植物材料的类群组成，即Ruminococcacae，Christensenellaceae和Lachnospiraceae。有趣的是，迄今为止，Christensenallaceae家族仅在人类中观察到。然而，在P. japonica中发现的Christensenellaceae操作分类单位属于该科内的不同分类分支。
Popillia japonica Newman (Coleoptera: Scarabaeidae) is a highly polyphagous invasive beetle originating from Japan. This insect is highly resilient and able to rapidly adapt to new vegetation. Insect-associated microorganisms can play important roles in insect physiology, helping their hosts to adapt to changing conditions and potentially contributing to an insect's invasive potential. Such symbiotic bacteria can be part of a core microbiota that is stably transmitted throughout the host's life cycle or selectively recruited from the environment at each developmental stage. The aim of this study was to investigate the origin, stability and turnover of the bacterial communities associated with an invasive population of P. japonica from Italy. Our results demonstrate that soil microbes represent an important source of gut bacteria for P. japonica larvae, but as the insect develops, its gut microbiota richness and diversity decreased substantially, paralleled by changes in community composition. Notably, only 16.75% of the soil bacteria present in larvae are maintained until the adult stage. We further identified the micro-environments of different gut sections as an important factor shaping microbiota composition in this species, likely due to differences in pH, oxygen availability and redox potential. In addition, P. japonica also harboured a stable bacterial community across all developmental stages, consisting of taxa well known for the degradation of plant material, namely the families Ruminococcacae, Christensenellaceae and Lachnospiraceae. Interestingly, the family Christensenallaceae had so far been observed exclusively in humans. However, the Christensenellaceae operational taxonomic units found in P. japonica belong to different taxonomic clades within this family.