Studies of hamster cardiac myofibrillogenesis in vivo with antibodies to spectrin, desmin, and alpha-actinin.
Studies of hamster cardiac myofibrillogenesis in vivo with antibodies to spectrin, desmin, and alpha-actinin.
复制标题
使用血影蛋白、结蛋白和α-辅肌动蛋白抗体对仓鼠心肌原纤维发生进行体内研究。
DOI：
10.1002/aja.1001910109
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Lemanski,LF
中科院分区：
文献类型：
--
作者：
Messina,DA;Lemanski,LF
谱蛋白是一种细胞骨架膜蛋白家族，在过去的十年中引起了人们的极大兴趣。在本研究中，我们利用免疫组织化学、形态学和电泳技术来评估谱蛋白在哺乳动物心脏组织发育过程中的可能功能。针对α -肌动蛋白和desmin产生的抗体也用于鉴定与谱蛋白分布变化有关的肌原纤维和中间纤维。Spectrin定位于肌原纤维前仓鼠心肌细胞的肌膜（第8天，性交后），并在整个发育过程中与细胞膜相关。染色模式起初有些弥漫性，但最终在性交后第13天细胞边缘变得清晰。第二个更深刻的变化发生在新生期，此时幽灵蛋白开始出现在肌质中。它表现为有规律的内陷，最初是弥漫性的，最终到达成人肌肉Z带周围的位置。谱蛋白分布的变化与T小管的出现在时间上是一致的，T小管是位于成人心脏Z波段的肉瘤内陷。因此，谱蛋白可能作为一种引导机制，引导T小管在哺乳动物心脏组织Z盘周围的正确定位。尽管spectrin似乎并不直接与早期肌原纤维相互作用，但它可能有助于T小管的正确排列，并通过将其包裹并附着在肌膜上来稳定整个收缩装置。
The spectrins are a family of cytoskeletal‐membrane proteins that have generated much interest in the past decade. In the present study, we utilized immunohistochemical, morphological, and electrophoretic techniques to assess the possible function(s) of spectrin in mammalian cardiac tissue during development. Antibodies generated against alpha‐actinin and desmin were also employed to identify myofibrils and intermediate filaments in relation to changes in the distribution of spectrin. Spectrin is localized along the sarcolemma of pre‐myofibrillar hamster cardiac myocytes (day 8, postcoitum) and remains associated with the cell membrane throughout development. The staining pattern is somewhat diffuse at first, but eventually the cell margin becomes clearly defined by day 13 postcoitum. A second, more profound change in the distribution of spectrin occurs during the newborn stage, when spectrin begins to appear in the sarcoplasm. It appears as regularly speced invaginations that are diffuse at first, eventually attaining a position around the Z‐bands of adult muscle. The change in the distribution of spectrin coincides temporally with the appearance of T‐tubules, which are sarcolmmal invaginations that reside at the Z‐bands of adult heart. Thus, spectrin may act as a guidance mechanism for the proper postioning of T‐tubules around the Z‐discs of mammalian cardiac tissue. Although spectrin does not appear to interact directly with early myofibrils it may assist in the proper alignment of T‐tubules and, in doing so, act to stabilize the entire contractile apparatus by enveloping it and attaching it to the sarcolemma.