Binding of plasma fibronectin to the surfaces of BHK cells in suspension at 4 degrees C.
Binding of plasma fibronectin to the surfaces of BHK cells in suspension at 4 degrees C.
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4°C 下，血浆纤连蛋白与悬浮液中的 BHK 细胞表面结合。
DOI：
10.1016/0014-4827(82)90398-6
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发表时间：
1982
影响因子：
3.7
通讯作者：
Phan,TV
中科院分区：
文献类型：
--
作者：
Grinnell,F;Lang,BR;Phan,TV
如果在 4 °C 下进行结合孵育，则通过几种不同的标准显示血浆纤连蛋白可与悬浮的 BHK 细胞结合。在间接免疫荧光实验中，纤连蛋白在 4 °C 下与悬浮的 BHK 细胞结合，在整个细胞表面呈点状分布。然而，在 37°C 下与纤连蛋白一起孵育的细胞上几乎没有检测到结合。在 4°C 下与细胞结合的纤连蛋白具有功能活性，因为这些细胞随后能够在无蛋白培养基中的组织培养皿上扩散，这与在 37°C 下与纤连蛋白预孵育或在不存在纤连蛋白的情况下的细胞不同。此外，可溶性纤连蛋白和纤连蛋白包被珠的细胞表面受体似乎相似，因为在 4°C 下与纤连蛋白预孵育的细胞随后比对照细胞结合更少的纤连蛋白包被珠。在放射性标记纤连蛋白的生化研究中，纤连蛋白与细胞的结合显示出随着孵育时间长达 4 小时而增加。在与未标记的纤连蛋白的竞争实验中，放射性标记的纤连蛋白的结合的30%可以被抑制。
Plasma fibronectin is shown by several different criteria to bind to suspended BHK cells if the binding incubations are carried out at 4 °C. In indirect immunofluorescence experiments, fibronectin bound to suspended BHK cells at 4 °C in a punctate distribution over the entire cell surfaces. Little binding, however, was detected on cells incubated with fibronectin at 37 °C. The fibronectin bound to the cells at 4 °C was functionally active, since these cells subsequently were able to spread on tissue culture dishes in protein-free medium, unlike cells preincubated with fibronectin at 37 °C or in the absence of fibronectin. Also, the cell surface receptors for soluble fibronectin and fibronectin-coated beads appeared to be similar, since cells preincubated with fibronectin at 4 °C subsequently bound fewer fibronectin-coated beads than control cells. In biochemical studies with radiolabeled fibronectin, binding of fibronectin to the cells was shown to increase with incubation time up to 4 h. In competition experiments with unlabeled fibronectin, 30% of the binding of radiolabeled fibronectin could be inhibited.