High-sensitivity Orbitrap mass analysis of intact macromolecular assemblies
High-sensitivity Orbitrap mass analysis of intact macromolecular assemblies
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DOI：
10.1038/nmeth.2208
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发表时间：
2012-11-01
期刊：
影响因子：
48
通讯作者：
Heck, Albert J. R.
中科院分区：
文献类型：
--
作者：
Rose, Rebecca J.;Damoc, Eugen;Heck, Albert J. R.
通过质谱分析天然状态下的完整蛋白质组装体提供了关于其生物化学和生物物理性质的丰富信息。在这里，我们表明，轨道阱质量分析仪可用于测量蛋白质组件的分子量接近一兆道尔顿的灵敏度下降到检测的单离子。微小的仪器修改，使各种蛋白质组装的测量具有出色的质谱分辨率。
The analysis of intact protein assemblies in native-like states by mass spectrometry offers a wealth of information on their biochemical and biophysical properties. Here we show that the Orbitrap mass analyzer can be used to measure protein assemblies of molecular weights approaching one megadalton with sensitivity down to the detection of single ions. Minor instrumental modifications enabled the measurement of various protein assemblies with outstanding mass-spectral resolution.