35-GHz (Q-band) saturation transfer electron paramagnetic resonance studies of rotational diffusion.
35-GHz (Q-band) saturation transfer electron paramagnetic resonance studies of rotational diffusion.
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旋转扩散的 35 GHz（Q 波段）饱和转移电子顺磁共振研究。
DOI：
10.1021/bi00513a025
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Hyde,JS
中科院分区：
文献类型：
--
作者：
Johnson,ME;Hyde,JS
Michael E. Johnson* and James S. Hyde abstract: The extension of saturation transferelectron the slow anisotropic motions believed to occur in many bioparamagnetic resonance spectroscopy (ST-EPR) to an ob- molecular systems. The spectral characteristics and the effects servational frequency of 35 GHz (Qband) is described. At of various instrumental settings are described in detail for a this frequency the spectral resolution is greatly enhanced over model system of spin-labeled hemoglobin in water-glycerol that afforded atthe 9.5-GHz (X-band) frequency used in most solutions. Several spectral parameters are defined, and their of the ST-EPR studies published to date. Thus, Q-band op- potential use in monitoring varioustypes of anisotropic motion eration may provide an approach for the detailed analysis of is considered. e application of spin-label rapid-passage saturation transfer EPR (ST-EPR) 1 to various biomolecular studies has shown a marked and continuing increase since its introduction by Hyde & Thomas (1973). Very recent applications include the use of several spin probes to studymotional behavior within the hydrocarbon region of model membrane systems (Delmelle et al., 1980; Marsh, 1980), the use of a spin-label NAD+ derivative to study lactate dehydrogenase solution conforma-tion (Trommer & Gloggler, 1979), studies of spectrin interactions with the erythrocyte membrane (Fung et al., 1979; Lemaigre-Dubreuil et al., 1980), studies of F-actin rotational dynamics (Thomas et al., 1979), studies of the hydrodynamic properties of glyceraldehyde-3-phosphate dehydrogenase (Beth et al., 1979), studies of protein-protein interactions and rho-dopsin rotational motionin membranes (Kusumi et al., 1978; Baroin et al., 1979; Davoust et al., 1980), and comparative studies of membrane fluidity for erythrocytes from normal and myotonic goats (Swift et al., 1980). Many of these and other applications havebeen discussed in recent reviews (Hyde, 1978; Hyde & Dalton, 1979; Hyde & Thomas, 1980). From the studies published to date, two limitations in the use of ST-EPRtechniques, as currently developed, are be-coming apparent:(1) ST-EPR spectra in a variety of biomolecular systems provide qualitative evidence for the existence of anisotropic motion, but quantitative analysis of such motion is quite difficult due to spectral overlap of spin states and turning points, 2 and (2) approximately 0.1 mL of solution is required for the conventional X-band flat cell, limiting the types of studies that can be performed with some systems. The problem of anisotropic motion in ST-EPR has been approached experimentally through the use of spin probe-thiourea adduct (Gaffney, 1979) and lipid bilayer (Delmelle et al., 1980) model systems. Extensive theoretical calculations of ST-EPR spectral behavior have also been reported for the two cases where the diffusion tensor is either coincident with, or orthogonal to, the label magnetic tensor (Robinson & Dalton, 1980). However
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