BLOOD EOSINOPHILS AND EOSINOPHIL-DERIVED PROTEINS IN ALLERGIC-ASTHMA
BLOOD EOSINOPHILS AND EOSINOPHIL-DERIVED PROTEINS IN ALLERGIC-ASTHMA
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DOI：
10.1016/0091-6749(89)90391-6
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发表时间：
1989-12-01
影响因子：
14.2
通讯作者：
KAY, AB
中科院分区：
文献类型：
--
作者：
DURHAM, SR;LOEGERING, DA;KAY, AB
测定了12例特应性哮喘患者和23例正常对照者外周血中嗜酸性粒细胞（EOS）衍生蛋白、主要碱性蛋白（MBP）、EOS衍生神经毒素（EDN）、EOS过氧化物酶（EPO）、EOS阳离子蛋白（ECP）的含量及EOS计数。同样的测量进行了7名哮喘受试者与先前记录的双重（早期加晚期）哮喘反应后吸入乙酰甲胆碱和过敏原的挑战。与对照组相比，哮喘患者的EOSs（p <0.001）、MBP（p <0.01）、EDN（p < 0.01）和ECP（p > 0.03）升高，而EPO（p < 0.01）浓度降低。在乙酰甲胆碱和过敏原激发日，FEV 1、EOSs、MBP、EDN、EPO或ECP的基线测量值之间无显著差异。当过敏原攻击后这些变量的变化与乙酰甲胆碱攻击后的相应变化进行比较时，在0至60分钟或3小时时无显著差异，而EDN在0至60分钟或3小时时无显著差异。EPO（p <0.05）和ECP（p <0.025）在6 ~ 12小时相对升高，并伴有FEV 1的后期福尔斯下降（p < 0.001）。在24小时时，当MBP有小的相对增加（p <0.05）时，EOS（p < 0.025）升高。EOS在过敏性哮喘受试者中似乎被“激活”，并且在晚期哮喘反应期间可能发生进一步激活。
The concentrations of the eosinophil (EOS)-derived proteins, major basic protein (MBP), EOS-derived neurotoxin (EDN), EOS peroxidase (EPO), and EOS cationic protein (ECP), and EOS counts were measured in the peripheral blood of 12 atopic subjects with asthma and 23 normal control subjects. The same measurements were performed in seven subjects with asthma with previously documented dual (early plus late) asthmatic responses after inhalation challenges with methacholine and allergen. EOSs (p < 0.001), MBP (p < 0.01), EDN (p < 0.01), and ECP (p > 0.03) were elevated in the subjects with asthma compared with control subjects, whereas EPO (p < 0.01) concentrations were reduced. There were no significant differences between baseline measurements of FEV1, EOSs, MBP, EDN, EPO, or ECP on the methacholine- and allergen-challenge days. When the changes in these variables afer allergen challenge were compared with the corresponding changes after methacholine challenge, there were no significant differences at 0 to 60 minutes or at 3 hours, whereas EDN (p < 0.025), EPO (p < 0.05), and ECP (p < 0.025) were relatively increased at 6 to 12 hours and accompanied the late falls in FEV1 (p < 0.001). EOSs (p < 0.025) were elevated at 24 hours when there was a small relative increase in MBP (p < 0.05). EOSs appear to be "activated" in subjects with allergic asthma, and further activation may occur during late asthmatic responses.