The interaction of thermal tolerance with drug cytotoxicity in vitro.
The interaction of thermal tolerance with drug cytotoxicity in vitro.
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DOI：
10.1038/bjc.1979.75
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发表时间：
1979-04
影响因子：
8.8
通讯作者：
Bleehen, N M
中科院分区：
文献类型：
--
作者：
Morgan, J E;Honess, D J;Bleehen, N M
已经研究了体外预热EMT6细胞对其对43 ℃或37 ℃的细胞毒性剂的反应的影响。在40 ℃下预热3小时对随后在43 ℃下处理1小时产生可测量的保护（耐热性）。进一步发现该预热处理减少了在43 ℃下1小时期间存在的BLM和BCNU（药物耐受性）对细胞的杀伤。与此相反，没有这样的热诱导的药物耐受性与ADR。ADR的另一个作用是在毒性药物剂量下明显消除热诱导的热耐受性。然而，在这些条件下预热对任何这些药物在37 ℃下的后续细胞毒性没有影响。此外，发现在43 ° C下预热1小时使细胞在37 ° C下对BLM和BCNU毒性敏感，但防止ADR毒性。结果进行了讨论，在已知的机制，细胞杀死热和耐热性。
The effect of preheating EMT6 cells in vitro on their response to cytotoxic agents of either 43 degrees C or 37 degrees C has been investigated. Preheating for 3 h at 40 degrees C produced measurable protection (thermal tolerance) to subsequent treatment for 1 h at 43 degrees C. This preheat treatment was further found to reduce cell killing by BLM and BCNU (drug tolerance) present during 1 h at 43 degrees C. In contrast, no such heat-induced drug tolerance was seen with ADR. An additional effect with ADR was the apparent elimination of heat-induced thermal tolerance at toxic drug doses. However, preheating under these conditions had no effect on the subsequent cytotoxicity of any of these drugs at 37 degrees C. Also, preheating for 1 h at 43 degrees C was found to sensitize cells to BLM and BCNU toxicity at 37 degrees C but to protect against ADR toxicity. The results are discussed in relation to known mechanisms of cell killing by heat and of thermal tolerance.