ON SIZE OF ACTIVE SITE IN PROTEASES .I. PAPAIN
ON SIZE OF ACTIVE SITE IN PROTEASES .I. PAPAIN
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DOI：
10.1016/s0006-291x(67)80055-x
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发表时间：
1967-01-01
影响因子：
3.1
通讯作者：
BERGER, A
中科院分区：
文献类型：
--
作者：
SCHECHTER, I;BERGER, A
材料和方法。作为底物的丙氨酸肽的合成已经被描述过（Schechter和Berger， 1966a）。采用2倍结晶（Worthington）木瓜蛋白酶。反应条件见图3。通过定量纸电泳测定不同时间反应混合物的组成（Schechter and Berger, 1966b）。图2给出了两个例子。必要时，分离反应产物，用CP-A和/或LAP进一步降解确定其立体化学组成（Schechter和Berger~ 1966b）。例如：Alas （DLL-LL）中的分裂显示发生在指定的点上，因为它只给出了AIa2 (LL)，而没有
MATERIALS AND METHODS. The synthesis of the alanine peptides used as substrates has already been described (Schechter and Berger, 1966a). Papain, 2x crystallized (Worthington) was used. For reaction conditions see Fig. 3. The compositions of the reaction mixtures at different times were determined by quantitative paper electrophoresis (Schechter and Berger, 1966b). Two examples are given in Fig. 2. When necessary, the reaction products were isolated and their stereochemical composition determined by further degradation with CP-A and/or LAP (Schechter and Berger~ 1966b). For example: the split in Alas (DLL-LL) was shown to have occurred at the point indicated, since it gave AIa2 (LL) only, and no