Development of aptamer-modified SERS nano sensor and oligonucleotide chip to quantitatively detect melamine in milk with high sensitivity
Development of aptamer-modified SERS nano sensor and oligonucleotide chip to quantitatively detect melamine in milk with high sensitivity
复制标题
开发适配体修饰的SERS纳米传感器和寡核苷酸芯片高灵敏度定量检测牛奶中的三聚氰胺
DOI：
10.1016/j.snb.2015.12.089
复制
发表时间：
2016-06-02
影响因子：
8.4
通讯作者：
Liu, Jianzhi
中科院分区：
文献类型：
--
作者：
Dong, Ning;Hu, Yongjun;Liu, Jianzhi
在此，我们利用适体修饰的表面增强拉曼散射(SERS)纳米传感器和寡核苷酸芯片开发了一种新型的纳米传感器来检测牛奶中的微量三聚氰胺(M)。将拉曼标签和聚胸腺嘧啶(T)适配子分别连接到金纳米粒子上，制备了SERS纳米传感器。SERS纳米传感器可以高特异性地吸附在寡核苷酸芯片上，其中三聚氰胺分子和胸腺嘧啶分子之间可以通过多个氢键形成T-M-T结构。在测量中，芯片的拉曼信号强度随着溶液中三聚氰胺浓度的增加而增加。该方法检测三聚氰胺的最低限量为1.0ppt(1.0pgmL(-1))，远远低于最严格的安全限值。与以往的方法相比，该传感器的灵敏度提高了约100倍。该传感器具有检测成本低、样品前处理简单等优点。将该纳米传感器应用于实际牛奶样品中三聚氰胺的检测，获得了可靠、准确的结果。(C)2015爱思唯尔B.V.保留所有权利。
Herein, we developed a novel nanosenor to detect trace amount of melamine (M) in the milk using aptamer-modified surface-enhanced Raman scattering (SERS) nanosensor and oligonucleotide chip. The SERS nanosensor was prepared by attaching Raman tag and ploy-thymine (T) aptamer to the gold nanoparticles, respectively. SERS nanosensor can be absorbed to the oligonucleotide chip with a high specificity where a "T-M-T" structure via multi-hydrogen-bond could be formed between the melamine molecule and the thymine molecule. In the measurement, the Raman signal intensities obtained from the chip increase when the concentrations of melamine in solutions increase. The proposed method allows us to detect melamine with a limit of 1.0 ppt (1.0 pg mL(-1)), which is fall far below the strictest safety limit. Compared with the previous methods, the sensitivity of this sensor shows about 100-fold improvement. The advantages of the present sensor are its low detection cost and the simplified sample pretreatment. Furthermore, the reliable and enough accurate results have been obtained by the using of the proposed nanosensor in the assay of trace melamine in real milk sample. (C) 2015 Elsevier B.V. All rights reserved.