Recent transposition activity of Xenopus T2 family miniature inverted-repeat transposable elements
Recent transposition activity of Xenopus T2 family miniature inverted-repeat transposable elements
复制标题
DOI：
10.1007/s00438-010-0599-3
复制
发表时间：
2011-03-01
影响因子：
3.1
通讯作者：
Kawahara, Akira
中科院分区：
文献类型：
--
作者：
Hikosaka, Akira;Nishimura, Kazuki;Kawahara, Akira
为了研究热带爪蟾（Xenopus tropicalis） T2家族微型倒置重复转座元件（MITEs）最近的转座活性，我们分析了热带爪蟾T2家族3个亚家族（T2- a1、T2- c和T2- e）与MITE插入相关的种内多态性。在T2-A1（50%）和T2-C（60%）插入位点上观察到高频率的mite插入多态性，但在T2-E插入位点上没有发现（0%）。对收集数据的分析表明，T2-A1和T2-C亚科的成员目前可能在宿主物种中活跃。识别这些主动转位将有助于我们理解T2-A1和T2-C亚家族长期生存（超过数千万年）的机制。
To investigate the recent transposition activity of T2 family miniature inverted-repeat transposable elements (MITEs) in Xenopus tropicalis (Western clawed frog), we analyzed the intraspecific polymorphisms associated with MITE insertion in X. tropicalis for three subfamilies of the T2 family (T2-A1, T2-C, and T2-E). A high frequency of MITE-insertion polymorphisms was observed at the T2-A1 (50%) and T2-C insertion loci (60%), but none were noted at the T2-E insertion locus (0%). Analyses of the collected data indicated that members of the T2-A1 and T2-C subfamilies may be currently active in the host species. Identification of these active transpositions will help us in understanding the mechanisms underlying the long-term survival (over several tens of millions of years) of the T2-A1 and T2-C subfamilies.