Identification of genes for dimethyl sulfide production in bacteria in the gut of Atlantic Herring (Clupea harengus)
Identification of genes for dimethyl sulfide production in bacteria in the gut of Atlantic Herring (Clupea harengus)
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DOI：
10.1038/ismej.2009.93
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发表时间：
2010-01-01
期刊：
影响因子：
11
通讯作者：
Johnston, Andrew W. B.
中科院分区：
文献类型：
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作者：
Curson, Andrew R. J.;Sullivan, Matthew J.;Johnston, Andrew W. B.
浮游植物是含硫相容性溶质二甲基磺基丙酸盐（DMSP）的主要生产者。这些细胞被中型浮游动物消耗，而中型浮游动物又可能被海洋脊椎动物吃掉。从这样一种动物，大西洋鲱鱼鲱鱼的肠道，我们分离出菌株的γ-变形杆菌假单胞菌和嗜冷杆菌DMSP作为唯一的碳源，并产生环境重要的含硫挥发性二甲基硫醚（DMS）上生长。在这两种细菌属中，这种能力是由于先前鉴定的基因dddD，该基因指定了一种从DMSP中释放DMS的酶。通过在底物DMSP上预生长细胞来刺激DMS产生。这是第一次在任何脊椎动物的肠道微生物区系中鉴定出DMSP降解细菌及其相关基因。The ISME Journal（2010）4，144-146; doi：10.1038/ismej.2009.93; 2009年8月27日在线发表
Phytoplankton are the primary producers of the sulfur-containing compatible solute dimethylsulfoniopropionate (DMSP). These cells are consumed by mesozooplankton, which, in turn, may be eaten by marine vertebrates. From the gut of one such animal, the Atlantic Herring Clupea harengus, we isolated strains of the gamma-proteobacteria Pseudomonas and Psychrobacter that grew on DMSP as sole carbon source, and which produced the environmentally important sulfurous volatile dimethyl sulfide (DMS). In both bacterial genera, this ability was because of the previously identified gene dddD, which specifies an enzyme that liberates DMS from DMSP. DMS production was stimulated by pre-growth of cells on the substrate DMSP. This is the first identification of DMSP-degrading bacteria and their relevant genes in the gut microflora of any vertebrate. The ISME Journal (2010) 4, 144-146; doi:10.1038/ismej.2009.93; published online 27 August 2009