Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae.
Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae.
复制标题
编码盘尾丝虫感染性幼虫抗原的表达 cDNA 克隆的分离和表征。
DOI：
10.1172/jci113581
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Greene,BM
中科院分区：
文献类型：
--
作者：
Unnasch,TR;Gallin,MY;Soboslay,PT;Erttmann,KD;Greene,BM
分离表达盘尾丝虫感染性幼虫中发现的抗原的重组cDNA克隆。为了分离这些克隆，构建了O.用抗感染性幼虫的抗血清筛选肠扭转RNA。进一步表征命名为λ RAL-1的一个克隆。由λ RAL-1产生的重组抗原刺激来自O.肠扭转感染了人类Lambda RAL-1来源于1450个碱基的信息，其编码成年O.肠扭转插入的λ RAL-1的DNA含有1008 bp的开放阅读框。由该开放阅读框预测的氨基酸序列包含序列KKPEDWD的三个重复。克隆如λ RAL-1的鉴定将提供足够数量的纯化抗原，以开始研究对寄生虫感染形式的免疫应答和探索免疫力的发展。图片
The isolation of recombinant cDNA clones expressing antigens found in Onchocerca volvulus infective larvae is described. To isolate such clones, an expression cDNA library constructed from adult O. volvulus RNA was screened with antiserum raised against infective larvae. One clone, designated lambda RAL-1 was characterized further. The recombinant antigen produced by lambda RAL-1 stimulates proliferation of peripheral blood mononuclear cells from O. volvulus infected humans. Lambda RAL-1 is derived from a 1450 bases message that encodes a protein with an apparent molecular weight of 42,000 in adult O. volvulus. The inserted DNA of lambda RAL-1 contains an open reading frame of 1008 bp. The amino acid sequence predicted by this open reading frame contains three repeats of the sequence KKPEDWD. The identification of clones such as lambda RAL-1 will provide quantities of purified antigens sufficient to begin to study the immune response to and explore the development of immunity against the infectious form of the parasite.Images