Diversity of culturable sulfidogenic bacteria in two oil-water separation tanks in the north-eastern oil fields of India
Diversity of culturable sulfidogenic bacteria in two oil-water separation tanks in the north-eastern oil fields of India
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DOI：
10.1016/j.anaerobe.2009.04.005
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发表时间：
2010-02-01
期刊：
影响因子：
2.3
通讯作者：
Lal, Banwari
中科院分区：
文献类型：
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作者：
Agrawal, Akhil;Vanbroekhoven, Karolien;Lal, Banwari
在印度东北部的陆上油田的生产沃茨中的硫化物群落进行了研究，使用培养的方法。将生产水样接种到硫酸盐还原菌（SRB）和硫代硫酸盐还原菌（TRB）选择性培养基中。从两个生产水箱中获得的样品中活的产硫微生物的总数约为105 MPN ml（-1）（每ml的最可能数）。大多数分离物是耐热的，可以在40至45摄氏度之间生长。TRB产生的硫化氢显著高于SRB。根据16 S rRNA基因测序，将分离株分为9个不同的系统发育型。系统发育分析表明，大多数SRB隶属于变形菌门，包括革兰氏阴性细菌，属于脱硫弧菌属，脱硫微菌属和脱硫菌属。然而，5个菌株分组与脱硫肠状菌被发现是革兰氏阳性SRB。大多数硫代硫酸盐还原分离株属于厚壁菌门，包括梭菌属和梭杆菌属，也属于变形菌门，包括肠杆菌属和柠檬酸杆菌属。在生产水样中，梭菌属（69%）和脱硫弧菌属（53%）的系统发育型占优势。这项研究表明，在印度东北部油田的油水分离罐的酸化介导的腐蚀中起关键作用的TRB和SRB的多样性。(C)2009爱思唯尔有限公司保留所有权利。
Sulfidogenic communities in the production waters of onshore oil fields in north-eastern India were examined using a culturing approach. Production water samples were inoculated into medium selective for Sulfate reducing bacteria (SRB) and Thiosulfate Reducing Bacteria (TRB). The total number of viable sulfidogenic microorganisms in the samples obtained from the two production water tanks was approximately 105 MPN ml(-1) (most probable number per ml). Most of the isolates were thermo-tolerant and could be grown between 40 and 45 degrees C. Hydrogen sulfide production by TRB was significantly higher than by SRB. Based on 16S rRNA gene sequencing, the isolates were grouped in nine different phylotypes. Phylogenetic analysis indicated that most of the SRB were affiliated with the phylum Proteobacteria, encompassing Gram-negative bacteria, belonging to the genera Desulfovibrio, Desulfomicrobium, and Desulfobulbus. However, five isolates grouped with the genus Desulfotomaculum were found to be gram-positive SRB. Most of the thiosulfate reducing isolates was affiliated with the phylum Firmicutes, including Clostridium and Fusibacter and also with the phylum Proteobacteria, including the genera Enterobacter and Citrobacter. Phylotypes related to Clostridium (69%) and Desulfovibrio (53%) dominated the community in the production water samples. This study demonstrates the diversity of the TRB and SRB that play a critical role in the souring mediated corrosion of the oil-water separation tanks in the north-eastern India oil fields. (C) 2009 Elsevier Ltd. All rights reserved.