CELL LINEAGES OF EMBRYO OF NEMATODE CAENORHABDITIS-ELEGANS
CELL LINEAGES OF EMBRYO OF NEMATODE CAENORHABDITIS-ELEGANS
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DOI：
10.1073/pnas.75.1.376
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
VONEHRENSTEIN, G
中科院分区：
文献类型：
--
作者：
DEPPE, U;SCHIERENBERG, E;VONEHRENSTEIN, G
自由生活的土壤线虫秀丽隐杆线虫的胚胎发生在孵化时产生具有约550个细胞的幼虫。我们通过追踪活胚胎中单个细胞的分裂，确定了 182 个细胞的谱系。卵子卵裂分裂的不变模式会产生一组干细胞。这些干细胞是六种干细胞谱系的创始人。每个谱系都有自己的时钟，即同步细胞分裂的自主节奏。尽管存在广泛的细胞重排，但节律仍得以维持。细胞谱系的细胞分裂的速率和方向在个体之间基本上是不变的。因此，细胞的命运似乎主要取决于它们的谱系历史。卵子中干细胞起源位点的前部位置与细胞周期时钟的速率相关，这表明未分裂卵子的细胞内预编程。我们使用了一种技术，可以在盖玻片下的母体之外进行正常的胚胎发生，从受精卵到孵化。使用诺马斯基干涉光学和高分辨率视频记录在显微镜下跟踪胚胎发生。
Embryogenesis of the free-living soil nematode Caenorhabditis elegans produces a juvenile having about 550 cells at hatching. We have determined the lineages of 182 cells by tracing the divisions of individual cells in living embryos. An invariant pattern of cleavage divisions of the egg generates a set of stem cells. These stem cells are the founders of six stem cell lineages. Each lineage has its own clock--i.e., an autonomous rhythm of synchronous cell divisions. The rhythms are maintained in spite of extensive cellular rearrangement. The rate and the orientation of the cell divisions of the cell lineages are essentially invariant among individuals. Thus, the destiny of cells seems to depend primarily on their lineage history. The anterior position of the site of origin of the stem cells in the egg relates to the rate of the cell cycle clock, suggesting intracellular preprogramming of the uncleaved egg. We used a technique that allows normal embryogenesis, from the fertilized egg to hatching, outside the parent under a cover glass. Embryogenesis was followed microscopically with Nomarski interference optics and high-resolution video recording.