NEW DETECTION AND SEPARATION METHOD FOR AMINO-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
NEW DETECTION AND SEPARATION METHOD FOR AMINO-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0021-9673(00)81650-7
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
ASAI, K
中科院分区：
文献类型：
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作者：
ISHIDA, Y;FUJITA, T;ASAI, K
建立了一个同时荧光检测伯胺和仲胺的简单系统。研究了次氯酸钠-邻苯二甲醛两段反应技术中仲胺荧光的最佳条件，并对切换流动法（次氯酸钠只加入仲胺中）和非切换流动法（次氯酸钠加入所有氨基酸中）进行了比较。结果表明，无切换流量法保证了高效率和高可靠性。将该体系应用于氨基酸的测定，获得了满意的结果。
A simple system for simultaneous fluorescence detection of primary and secondary amines has been developed. The optimum conditions for fluorescing secondary amines in the two-stage reaction technique using sodium hypochlorite ando-phthalaldehyde have been studied, and then the switching flow method (sodium hypochlorite is added only to secondary amines) and the non-switching flow method (sodium hypochlorite is added to all the amino acids) have been compared. It was concluded that the non-switching flow method ensures high efficiency and reliability. This newly-developed system was applied to the detection of amino acids and gave satisfactory results.