Novel biomolecular information in rotenone-induced cellular model of Parkinson's disease
Novel biomolecular information in rotenone-induced cellular model of Parkinson's disease
复制标题
鱼藤酮诱导的帕金森病细胞模型的新生物分子信息
DOI：
10.1016/j.gene.2018.01.023
复制
发表时间：
2018-03-20
期刊：
影响因子：
3.5
通讯作者：
Tao, E.
中科院分区：
文献类型：
--
作者：
Lin, D.;Liang, Y.;Tao, E.
为了揭示帕金森病（Parkinson's disease，PD）的致病基因或分子病理过程，我们应用基因芯片技术对鱼藤酮诱导的细胞PD模型进行了研究。与对照组相比，根据一定的标准筛选出2174个基因在鱼藤酮诱导组中表达差异。GO分析和途径分析显示，与细胞周期、凋亡过程、细胞器融合、线粒体损伤、内质网应激等生物学过程相关的基因显著富集，在这些显著的DE基因中，筛选出一些与细胞周期和内质网蛋白质加工相关的基因。PPI网络分析表明，内质网蛋白质生成过程中DEG之间的相互作用关系清晰可见。因此，我们认为EDEM 1、ATF4、TRAF2基因可能在帕金森病的蛋白质错误折叠过程中发挥重要作用，这些新发现的基因可能成为帕金森病未来的研究热点和治疗靶点。
In order to uncover the remarkable pathogenic genes or molecular pathological process in Parkinson's disease (PD), we employed a microarray analysis upon the cellular PD model induced by rotenone. Compared to the control group, 2174 genes were screened out to be expressed differently in the rotenone-induced group by certain criterion. GO analysis and the pathways analysis showed the significant enrichment of genes that were associated with the biological process of cell cycle, apoptotic process, organelle fusion, mitochondrial lesion, endoplasmic reticulum stress and so on. Among these significant DE genes, some were sorted out to be involved in cell cycle and protein processing in endoplasmic reticulum. As the PPI network analysis showed, the interaction relationship of the DEGs involved in the process of protein generation in endoplasmic reticulum(ER) was clearly showed up. As a prediction, we emphasized the genes EDEM1, ATF4, TRAF2 might play central roles in the protein misfolding process during the progression of Parkinson's disease and these new-found genes might be the future research focus and therapeutic targets in PD.