Detection of cathepsin B, cathepsin L, cystatin C, urokinase plasminogen activator and urokinase plasminogen activator receptor in the sera of lung cancer patients
Detection of cathepsin B, cathepsin L, cystatin C, urokinase plasminogen activator and urokinase plasminogen activator receptor in the sera of lung cancer patients
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DOI：
10.3892/ol.2011.302
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发表时间：
2011-07-01
期刊：
影响因子：
2.9
通讯作者：
Lu, Guohua
中科院分区：
文献类型：
--
作者：
Chen, Qingyong;Fei, Jun;Lu, Guohua
本研究旨在采用ELISA法检测肺癌患者血清中组织蛋白酶B（cath B）、组织蛋白酶L（cath L）、胱抑素C、尿激酶型纤溶酶原激活物（u-PA）和尿激酶型纤溶酶原激活物受体（u-PAR）的水平，并与健康对照组进行比较。同时分析其与临床病理预后的关系。该研究包括30名健康志愿者和105名肺癌患者。收集血液样品并使用ELISA进行cath B、cath L、cystatin C、u-PA和u-PAR的测量。结果显示，患者组cath B、cath L、cystatin C、u-PA和u-PAR水平均显著高于健康对照组。cath B与淋巴结转移有显著相关性，u-PAR与淋巴结转移无显著相关性。其他参数无显著性差异。值得注意的是，与具有胱抑素C（+）/cath B（-）或具有胱抑素C（-）/cath B（-）的患者相比，具有高胱抑素C和高cath B水平组合的患者具有显著较低的存活概率。类似地，具有高u-PA和u-PAR组合的患者经历显著较短的存活。单因素分析显示，cath B、u-PAR、淋巴结转移、分期和分级与生存有关。多因素考克斯分析结果显示，cath B、u-PAR和淋巴结转移可能是肺癌患者的独立预后因素。
The present study aimed to determine the levels of cathepsin B (cath B), cathepsin L (cath L), cystatin C, urokinase plasminogen activator (u-PA) and urokinase plasminogen activator receptor (u-PAR) in the sera of patients with lung cancer compared to healthy controls using ELISA. Concomitantly, the relationship between the components and clinicopathological prognosis was analyzed. The study included 30 healthy volunteers and 105 lung cancer patients. Blood samples were collected and cath B, cath L, cystatin C, u-PA and u-PAR measurements were made using ELISA. Results showed that the levels of cath B, cath L, cystatin C, u-PA and u-PAR were significantly higher in the patient group compared to the healthy controls. The significance was marked for cath B and mild for u-PAR in correlation with lymph node metastasis. There was no significance for other parameters. Notably, patients with a combination of high cystatin C and high cath B levels had significantly lower survival probability as compared to those with cystatin C(+)/cath B(-) or with cystatin C(-)/cath B(-), Similarly, patients with a combination of high u-PA and u-PAR experienced significantly shorter survival., Furthermore, the univariate analysis revealed that cath B, u-PAR, lymph node metastases, stage and grade were related to survival. However, findings of the multivariate Cox analysis indicated that the sera levels of cath B, u-PAR and lymph node metastases may serve as independent prognostic variables in patients with lung cancer.