Oxygen enhancement of radiation induced lethality is greatly reduced in glutathione deficient human fibroblasts.
Oxygen enhancement of radiation induced lethality is greatly reduced in glutathione deficient human fibroblasts.
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在谷胱甘肽缺乏的人成纤维细胞中，氧气增强辐射诱导的致死率大大降低。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
László Révész
中科院分区：
文献类型：
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作者：
Deschavanne Pj;J. Midander;Margareta Edgren;A. Larsson;E. Malaise;László Révész
研究了基因确定的谷胱甘肽 (GSH) 缺乏的人成纤维细胞在氧气或无氧氩气中用 X 射线照射后的体外克隆存活情况。没有 GSH 缺乏的遗传相关成纤维细胞用作对照。两种细胞系的有氧存活曲线相似。与有氧存活曲线相比，GSH缺陷细胞的缺氧存活曲线表明氧增强比(OER)约为1.5。通过比较对照细胞系的有氧和缺氧生存曲线，计算出 OER 约为 2.9。讨论结果支持了以下理论：氧和谷胱甘肽竞争关键分子中的辐射诱导自由基，前者不可逆地固定，后者修复辐射损伤。
The in vitro clonogenic survival of human fibroblasts with a genetically defined glutathione (GSH) deficiency was studied after irradiation with X-rays in oxygen or in oxygen free argon. Genetically related fibroblasts without GSH deficiency were used as a control. The oxic survival curve of both cell lines was similar. In comparison to the oxic survival curve, the anoxic survival curve of GSH deficient cells indicated an oxygen enhancement ratio (OER) approximately 1.5. An OER approximately 2.9 was calculated in comparing the oxic and anoxic survival curves of the control cell line. The results were discussed in support of the theory according to which oxygen and GSH compete for radiation induced radicals in key molecules, the former irreversibly fixing and the latter repairing the radiation damage.