Detection of scrapie-associated fibrils (SAF) and SAF proteins from scrapie-affected sheep.
Detection of scrapie-associated fibrils (SAF) and SAF proteins from scrapie-affected sheep.
复制标题
检测受瘙痒病影响的绵羊的瘙痒病相关原纤维 (SAF) 和 SAF 蛋白。
DOI：
10.1093/infdis/156.1.36
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Wisniewski,HM
中科院分区：
文献类型：
--
作者：
Rubenstein,R;Merz,PA;Kascsak,RJ;Carp,RI;Scalici,CL;Fama,CL;Wisniewski,HM
羊瘙痒症相关的纤维（SAF）进行了检测，通过负染电子显微镜在脑（通过两种不同的隔离程序）和脾脏的羊自然和实验感染羊瘙痒症。虽然SAF的数量在不同的情况下，SAF的产量从自然感染的羊比从实验感染的羊脑。通过银染和Western印迹分析，在大多数实验感染绵羊的脑样品中检测到SAF特异性蛋白酶抗性蛋白（PrPs）。然而，由于SAP的产量较低，因此只能在有限数量的绵羊瘙痒症自然病例中检测到Pr Ps。来自绵羊SAF的PrPs在生物化学和抗原性上与感染非常规试剂的其他物种的PrPs相似。本研究进一步确立了SAF和PrPs与天然或实验诱导的瘙痒病在其天然宿主中的独特关联。
Scrapie-associated fibrils (SAF) were detected by negative-stain electron microscopy in the brains (by two different isolation procedures) and spleens of sheep naturally and experimentally infected with scrapie. Although the numbers of SAF varied from case to case, the yield of SAF from brains of naturally affected sheep was lower than that from experimentally affected sheep. SAF-specific, protease-resistant proteins (PrPs) were detected by silver staining and western blot analysis in most samples of brain from experimentally affected sheep. Pr Ps, however, could be detected in only a limited number of natural cases of sheep scrapie because of the lower yields of SAP. PrPs from sheep SAF appear biochemically and antigenically similar to PrPs from other species infected with unconventional agents. This study further establishes the unique association of SAF and PrPs with natural or experimentally induced scrapie in its natural host.