Compartmentation of deoxypyrimidine nucleotides for nuclear DNA replication in S phase mammalian cells.
Compartmentation of deoxypyrimidine nucleotides for nuclear DNA replication in S phase mammalian cells.
复制标题
S 期哺乳动物细胞中用于核 DNA 复制的脱氧嘧啶核苷酸的区室。
DOI：
10.1002/jmr.300020205
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Reddy,GP
中科院分区：
文献类型：
--
作者：
Reddy,GP
在存在外源 3H-dUrd 的情况下，S 期中国仓鼠胚胎成纤维细胞中的 DNA 合成显示，标记的前体在大的未标记的细胞内前体库中进行了很少的稀释。完全混合预计比活度比测量值低 10 倍。这与外源 3 H-dUrd 的 80% 放射性出现在核质体中的发现相结合，意味着来自外源 3 H-dUrd 的 3 H-dUMP 和 3 H-dTTP 不与内源细胞质库自由混合。
DNA synthesis in S phase Chinese hamster embryo fibroblast cells in the presence of exogenous3H‐dUrd shows incorporation of the labeled precursor with very little dilution by the large unlabeled intracellular precursor pools. Full mixing would predict a specific activity 10‐fold less than that measured. This coupled with the finding that 80% of the radioactivity from the exogenous3H‐dUrd appears in the karyoplasts implies a compartmentation where3H‐dUMP and3H‐dTTP derived from exogenous3H‐dUrd do not mix freely with endogenous cytoplasmic pools.