Plasticity of Interstitial Cells of Cajal: A Study in the Small Intestine of Adult Guinea Pigs
Plasticity of Interstitial Cells of Cajal: A Study in the Small Intestine of Adult Guinea Pigs
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DOI：
10.1002/ar.20928
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发表时间：
2009-07-01
影响因子：
2
通讯作者：
Zhou, De-Shan
中科院分区：
文献类型：
--
作者：
Mei, Feng;Han, Juan;Zhou, De-Shan
Although it is well known that the reduction of interstitial cells of Cajal (ICCs) is associated with several gastrointestinal motility disorders in clinic, it is unknown whether the mature ICCs still have an active plasticity in adult mammals. This study focused on the issues of the reduction of ICCs during Imatinib administration and the recovery of ICCs following drug withdrawal in the small intestine of adult guinea pigs. ICCs were revealed by immunofluoreseence on whole mount preparations with anti-Kit, a-smooth muscle actin, (alpha-SMA), and 5-bromo-2'-deoxyuridine (BrdU) antibodies. Moreover, the occurrence of apoptosis was also assayed. Imatinib treatment led to a gradual reduction of ICCs in number around the myenteric plexus and deep muscular plexus, which was dependent on the time but no apoptosis of ICCs was detected with the TUNEL method. During Imatinib treatment, some ICC-like cells were double labeled for Kit and alpha-SMA and a few ICC-like cells were only stained with alpha-SMA. When Imatinib was discontinued, the number of ICCs recovered to normal within 32 days. During this time, some proliferating ICCs were demonstrated by double labeling with Kit and BrdU antibodies. Our results indicated that Kit signaling was essential for the maintenance of survival and proliferation of the mature ICCs in the small intestine of adult guinea pigs. Moreover, ICCs might trans-differentiate to a type of alpha-SMA(+) cells, perhaps a phenotype of smooth muscle cells, when there is a loss-of-function of Kit. Anat Rec, 292:985993, 2009. (C) 2009 Wiley-Liss, Inc.
