Development of immunochromatographic test systems for express detection of plant viruses
Development of immunochromatographic test systems for express detection of plant viruses
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DOI：
10.1134/s000368380902015x
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发表时间：
2009-03-01
影响因子：
0.8
通讯作者：
Atabekov, I. G.
中科院分区：
文献类型：
--
作者：
Byzova, N. A.;Safenkova, I. V.;Atabekov, I. G.
建立了检测球形香石竹斑驳病毒、菜豆温和花叶病毒、杆状烟草花叶病毒和丝状马铃薯X和Y病毒的快速免疫层析试纸条。使用具有针对病毒的固定化多克隆抗体和胶体金缀合抗体的多膜复合物（测试条）进行分析。免疫层析测试条被证明能够检测病毒的纯化制剂和在叶提取物中的感染植物的灵敏度从0.08到0.5 μ g/ml为10分钟。测试条可用于快速诊断植物病毒病在现场条件下。
Express immunochromatographic test-strip assays were developed for detection of five plant viruses varying in shape and size of virions: spherical carnation mottle virus, bean mild mosaic virus, rodshaped tobacco mosaic virus, and filamentous potato viruses X and Y. Multimembrane composites (test strips) with immobilized polyclonal antibodies against viruses and colloidal gold-conjugated antibodies were used for the analysis. The immunochromatographic test strips were shown to enable the detection of viruses both in purified preparations and in leaf extracts of infected plants with a sensitivity from 0.08 to 0.5 mu g/ml for 10 min. The test strips may be used for express diagnostics of plant virus diseases in field conditions.